Evidence map›Paper›PMID 38478224›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Isolation of Perivascular Mesenchymal Progenitor Cells from Human Adipose Tissue by Flow Cytometry.

Neelima Thottappillil, Mario A Gomez-Salazar, Mary Archer, Bruno Péault, Aaron W James

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Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
3.6field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 2 countries.

Neelima Thottappillil *Department of Pathology, Johns Hopkins University, Baltimore, USA, MD.
Mario A Gomez-Salazar *Department of Pathology, Johns Hopkins University, Baltimore, USA, MD.
Mary ArcherDepartment of Pathology, Johns Hopkins University, Baltimore, USA, MD.
Bruno PéaultCenter for Cardiovascular Research, University of Edinburgh, Edinburgh, UK.
Aaron W JamesDepartment of Pathology, Johns Hopkins University, Baltimore, USA, MD. awjames@jhmi.edu.
Johns Hopkins University · USUniversity of Edinburgh · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Perivascular cells represent an in vivo counterpart of mesenchymal stromal/stem cells that populate the outer layer of blood vessels. Pericytes in capillaries and microvessels and adventitial cells of large arteries and veins give rise to stem/progenitor cells when isolated and cultured in vitro. These cells have been considered candidate cell types for cell therapy. Adipose tissue, being highly vascularized, dispensable, and easily accessed, is a viable option to obtain perivascular cells for use in research and in clinical trials. Here, we describe our established protocol to extract perivascular cells from human fat through fluorescence-activated cell sorting, which allows for the isolation of defined populations of progenitor cells with high reproducibility.

Indexed as

Mesenchymal Stem CellsAdipose TissueCell DifferentiationFlow CytometryHumansPericytesReproducibility of ResultsAdipose tissueAdventitial cellCell therapyFlow cytometryMesenchymal stromal/stem cellPericytePerivascular stem cell

Identifiers

PMID38478224
OpenAlexW4392742024

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.