Evidence map›Paper›PMID 38478163›Full record

ArticleJournal of neurovirology2024

RNA-Seq time-course analysis of neural precursor cell transcriptome in response to herpes simplex Virus-1 infection.

Joel A Wood, Srilakshmi Chaparala, Cecilia Bantang, Ansuman Chattopadhyay, Maribeth A Wesesky, Paul R Kinchington, Vishwajit L Nimgaonkar, David C Bloom, Leonardo D'Aiuto

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of neurovirology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.2field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 3 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Joel A WoodWestern Psychiatric Institute and Clinic, Department of Psychiatry, University of Pittsburgh School of Medicine, 3811 O'Hara Street, 15213, Pittsburgh, PA, USA.
Srilakshmi ChaparalaMolecular Biology Information Service, Health Sciences Library System / Falk Library, University of Pittsburgh, M722 Alan Magee Scaife Hall / 3550 Terrace Street, 15261, Pittsburgh, PA, USA.
Cecilia BantangWestern Psychiatric Institute and Clinic, Department of Psychiatry, University of Pittsburgh School of Medicine, 3811 O'Hara Street, 15213, Pittsburgh, PA, USA.
Ansuman ChattopadhyayMolecular Biology Information Service, Health Sciences Library System / Falk Library, University of Pittsburgh, M722 Alan Magee Scaife Hall / 3550 Terrace Street, 15261, Pittsburgh, PA, USA.
Maribeth A WeseskyWestern Psychiatric Institute and Clinic, Department of Psychiatry, University of Pittsburgh School of Medicine, 3811 O'Hara Street, 15213, Pittsburgh, PA, USA.
Paul R KinchingtonDepartment of Ophthalmology, University of Pittsburgh, Suite 820, Eye & Ear Building, 203 Lothrop Street, 15213, Pittsburgh, PA, USA.
Vishwajit L NimgaonkarWestern Psychiatric Institute and Clinic, Department of Psychiatry, University of Pittsburgh School of Medicine, 3811 O'Hara Street, 15213, Pittsburgh, PA, USA.
David C BloomAcademic Research Building, Department of Molecular Genetics and Microbiology, University of Florida, 1200 Newell Drive, R2-231, 32610, Gainesville, FL, USA.
Leonardo D'AiutoWestern Psychiatric Institute and Clinic, Department of Psychiatry, University of Pittsburgh School of Medicine, 3811 O'Hara Street, 15213, Pittsburgh, PA, USA. daiutol@upmc.edu.ORCID 0000-0002-7102-2093
University of Pittsburgh · USUnited States Department of Veterans Affairs · USUniversity of Florida · US

Funding

Virus Production and Manipulation of Protein/Gene Expression ModuleP30EY008098 · NEI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI Yuanyuan Chen · 1989 to 2026
$17.8M
Molecular Genetics of HSV ReactivationR01AI048633 · NIAID · UNIVERSITY OF FLORIDA · PI BLOOM, DAVID C. · 2001 to 2023
$7.2M
A Neurobehavioral Family Study of SchizophreniaR01MH063480 · NIMH · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI NIMGAONKAR, VISHWAJIT LAXMIKANT · 2001 to 2018
$6.1M
BASIC MICROBIOLOGY &INFECTIOUS DISEASEST32AI007110 · NIAID · UNIVERSITY OF FLORIDA · PI Stephanie M Karst · 1985 to 2026
$4.3M
Effects of HSV-1 reactivation from latency on aspects of neural precursor cells neurogenesis and accumulation of Alzheimer's molecular hallmarksR01NS115082 · NINDS · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI BLOOM, DAVID C., D'AIUTO, LEONARDO · 2020 to 2024
$2.3M
Molecular studies of VZV infection, latency and reactivation in human neurons in-vitroR01AI122640 · NIAID · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI KINCHINGTON, PAUL R. · 2016 to 2020
$2.3M
Analysis of human-specific aspects of HSV-1 latency in human iPSC-derived neuronsR21NS096405 · NINDS · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI D'AIUTO, LEONARDO · 2016 to 2017
$428k
NEI NIH HHS 2P30EY008098-31NEI NIH HHS P30 EY008098NIAID NIH HHS 5R01AI048633-16NIAID NIH HHS 5R01AI122640-05NIAID NIH HHS 5T32AI007110-35NIAID NIH HHS R01 AI048633NIAID NIH HHS R01 AI122640NIAID NIH HHS T32 AI007110NIMH NIH HHS 5R01MH063480-14NIMH NIH HHS R01 MH063480NINDS NIH HHS 1R01NS115082-01A1NINDS NIH HHS 1R21NS096405-01A1NINDS NIH HHS R01 NS115082NINDS NIH HHS R21 NS096405SMRI 07R-1712
6 · The paper itself

Abstract

The neurogenic niches within the central nervous system serve as essential reservoirs for neural precursor cells (NPCs), playing a crucial role in neurogenesis. However, these NPCs are particularly vulnerable to infection by the herpes simplex virus 1 (HSV-1). In the present study, we investigated the changes in the transcriptome of NPCs in response to HSV-1 infection using bulk RNA-Seq, compared to those of uninfected samples, at different time points post infection and in the presence or absence of antivirals. The results showed that NPCs upon HSV-1 infection undergo a significant dysregulation of genes playing a crucial role in aspects of neurogenesis, including genes affecting NPC proliferation, migration, and differentiation. Our analysis revealed that the CREB signaling, which plays a crucial role in the regulation of neurogenesis and memory consolidation, was the most consistantly downregulated pathway, even in the presence of antivirals. Additionally, cholesterol biosynthesis was significantly downregulated in HSV-1-infected NPCs. The findings from this study, for the first time, offer insights into the intricate molecular mechanisms that underlie the neurogenesis impairment associated with HSV-1 infection.

Indexed as

Herpes SimplexHerpesvirus 1, HumanNeural Stem CellsNeurogenesisRNA-SeqTranscriptomeAnimalsAntiviral AgentsCell DifferentiationCell MovementCell ProliferationCholesterolCyclic AMP Response Element-Binding ProteinGene Expression RegulationMiceSignal TransductionAntiviral AgentsCholesterolCyclic AMP Response Element-Binding ProteinHerpes simplex virus (HSV)(hiPSCs)Human induced pluripotent stem cellsNeural precursor cellsNeurospheresRNA-SeqTime-course analysis

Identifiers

PMID38478163
PMCPMC11371869
OpenAlexW4392738985

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.