Evidence map›Paper›PMID 38478141›Full record

ArticleExperimental & applied acarology2024

Developing a method to rear Varroa destructor in vitro.

Brynn L Johnson, Cody Prouty, Cameron J Jack, Charles Stuhl, James D Ellis

Abstract read
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In one paragraph

Article in Experimental & applied acarology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Brynn L JohnsonHoney Bee Research and Extension Laboratory, Entomology and Nematology Department, University of Florida, 32611, Gainesville, FL, USA. brynnjohnson@ufl.edu.
Cody ProutyHoney Bee Research and Extension Laboratory, Entomology and Nematology Department, University of Florida, 32611, Gainesville, FL, USA.
Cameron J JackHoney Bee Research and Extension Laboratory, Entomology and Nematology Department, University of Florida, 32611, Gainesville, FL, USA.
Charles StuhlCenter for Medical, Agricultural and Veterinary Entomology, USDA-ARS, 32608, Gainesville, FL, USA.
James D EllisHoney Bee Research and Extension Laboratory, Entomology and Nematology Department, University of Florida, 32611, Gainesville, FL, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Varroa destructor is a significant mite pest of western honey bees (Apis mellifera). Developing a method to rear and maintain populations of V. destructor in vitro would provide year-round access to the mites, allowing scientists to study their biology, behavior, and control more rapidly. In this study, we determined the impact of various rearing parameters on V. destructor survival and reproduction in vitro. This was done by collecting V. destructor from colonies, placing them in gelatin capsules containing honey bee larvae, and manipulating the following conditions experimentally: rearing temperature, colony source of honey bee larva, behavioral/developmental stages of V. destructor and honey bee larva, and mite:bee larva ratio. Varroa destructor survival was significantly impacted by temperature, colony source of larvae and mite behavioral stage. In addition, V. destructor reproduction was significantly impacted by mite: larva ratio, larval developmental stage, colony source of larva, and temperature. The following conditions optimized mite survival and reproduction in vitro: using a 4:1 mite:larva ratio, beginning the study with late stage uncapped larvae, using mites collected from adult bees, maintaining the rearing temperature at 34.5° C, and screening larval colony source. Ultimately, this research can be used to improve V. destructor in vitro rearing programs.

Indexed as

LarvaVarroidaeAnimalsBeekeepingBeesReproductionTemperatureApis melliferaHoney beeIn vitro rearingVarroa destructor

Identifiers

PMID38478141

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.