ArticleThe Science of the total environment2024
Comparison of adsorption-extraction (AE) workflows for improved measurements of viral and bacterial nucleic acid in untreated wastewater.
Article in The Science of the total environment, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 19 citations in OpenAlex.
- Control of Brominated and Iodinated DBPs within a Drinking Water Distribution System with Granular Activated Carbon and Biological Filtration.Environmental science & technology · 2026Article
- Surveillance of Japanese encephalitis virus in piggery effluent and environmental samples: a complementary tool for outbreak detection.Applied and environmental microbiology · 2025Article
- Understanding microbial biomineralization at the molecular level: recent advances.World journal of microbiology & biotechnology · 2024Review
- Inhibition of microelement accumulation and disorder of saccharide and amino acid metabolism explain rice grain empty under dimethylarsinic acid stress.Plant cell reports · 2024Article
- Assessment of nucleic acid extraction protocols for antibiotic resistance genes (ARGs) quantification in aircraft wastewater.Human genomics · 2024Article
- Effect of BaWorld journal of microbiology & biotechnology · 2024Article
- Integrating bioprocess and metagenomics studies to enhance humic acid production from rice straw.World journal of microbiology & biotechnology · 2024Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 4 countries.
Funding
Abstract
The lack of standardized methods and large differences in virus concentration and extraction workflows have hampered Severe Acute Respiratory Syndrome (SARS-CoV-2) wastewater surveillance and data reporting practices. Numerous studies have shown that adsorption-extraction (AE) method holds promise, yet several uncertainties remain regarding the optimal AE workflow. Several procedural components may influence the recovered concentrations of target nucleic acid, including membrane types, homogenization instruments, speed and duration, and lysis buffer. In this study, 42 different AE workflows that varied these components were compared to determine the optimal workflow by quantifying endogenous SARS-CoV-2, human adenovirus 40/41 (HAdV 40/41), and a bacterial marker gene of fecal contamination (
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Registered trials
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