Evidence map›Paper›PMID 38475831›Full record

ArticleJournal of translational medicine2024

Investigation of the acute pathogenesis of spondyloarthritis/HLA-B27-associated anterior uveitis based on genome-wide association analysis and single-cell transcriptomics.

Shuming Chen, Weidi Huang, Qiaoqian Wan, Zichun Tang, Xie Li, Fang Zeng, Shuyan Zheng, Zhuo Li, Xiao Liu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of translational medicine, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
5.2field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 11 citations in OpenAlex.

  1. Review
  2. Frontiers in immunology · 2026
    Article
  3. Observational
  4. Article
  5. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Shuming ChenDepartment of Ophthalmology, The Second Xiangya Hospital, Central South University, Changsha, 410011, China.
Weidi HuangDepartment of Ophthalmology, The Second Xiangya Hospital, Central South University, Changsha, 410011, China.
Qiaoqian WanDepartment of Anaesthesiology, the Second Xiangya Hospital, Central South University, Changsha, 410011, China.
Zichun TangDepartment of Ophthalmology, The Second Xiangya Hospital, Central South University, Changsha, 410011, China.
Xie LiDepartment of Ophthalmology, The Second Xiangya Hospital, Central South University, Changsha, 410011, China.
Fang ZengDepartment of Ophthalmology, The Second Xiangya Hospital, Central South University, Changsha, 410011, China.
Shuyan ZhengDepartment of Ophthalmology, The Second Xiangya Hospital, Central South University, Changsha, 410011, China.
Zhuo LiDepartment of Ophthalmology, The Second Xiangya Hospital, Central South University, Changsha, 410011, China. LiZhuo75@csu.edu.cn.ORCID 0009-0004-0165-2569
Xiao LiuDepartment of Ophthalmology, The Second Xiangya Hospital, Central South University, Changsha, 410011, China. Liuxiao8@csu.edu.cn.ORCID 0000-0002-7352-2913
Central South University · CN

Funding

National Natural Science Foundation of China 82000924Natural Science Foundation of Hunan Province 2023JJ30770
6 · The paper itself

Abstract

backgroundPatients with spondyloarthritis (SpA)/HLA-B27-associated acute anterior uveitis (AAU) experience recurring acute flares, which pose significant visual and financial challenges. Despite established links between SpA and HLA-B27-associated AAU, the exact mechanism involved remains unclear, and further understanding is needed for effective prevention and treatment.

methodsTo investigate the acute pathogenesis of SpA/HLA-B27-associated AAU, Mendelian randomization (MR) and single-cell transcriptomic analyses were employed. The MR incorporated publicly available protein quantitative trait locus data from previous studies, along with genome-wide association study data from public databases. Causal relationships between plasma proteins and anterior uveitis were assessed using two-sample MR. Additionally, colocalization analysis was performed using Bayesian colocalization. Single-cell transcriptome analysis utilized the anterior uveitis dataset from the Gene Expression Omnibus (GEO) database. Dimensionality reduction, clustering, transcription factor analysis, pseudotime analysis, and cell communication analysis were subsequently conducted to explore the underlying mechanisms involved.

resultsMendelian randomization analysis revealed that circulating levels of AIF1 and VARS were significantly associated with a reduced risk of developing SpA/HLA-B27-associated AAU, with AIF1 showing a robust correlation with anterior uveitis onset. Colocalization analysis supported these findings. Single-cell transcriptome analysis showed predominant AIF1 expression in myeloid cells, which was notably lower in the HLA-B27-positive group. Pseudotime analysis revealed dendritic cell terminal positions in differentiation branches, accompanied by gradual decreases in AIF1 expression. Based on cell communication analysis, CD141

conclusionsAIF1 is essential for the pathogenesis of SpA/HLA-B27-associated AAU. Myeloid cell differentiation into DCs and decreased AIF1 levels are also pivotal in this process.

Indexed as

SpondylarthritisUveitis, AnteriorAcute DiseaseBayes TheoremGene Expression ProfilingGenome-Wide Association StudyHLA-B27 AntigenHumansHLA-B27 AntigenAnterior uveitisGeneticsMendelian randomizationSingle-cell transcriptome

Identifiers

PMID38475831
PMCPMC10936029
OpenAlexW4392683836

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.