Evidence map›Paper›PMID 38474548›Full record

ArticleMolecules (Basel, Switzerland)2024

Establishing an ANO1-Based Cell Model for High-Throughput Screening Targeting TRPV4 Regulators.

Kai Zheng, Jiang Hu, Cheng Hu, Xueying Liu, Yanyan Wang, Haojian Han, Wenzhu Xing, Liu Yang, Junran Zhang, Qiyuan Hong and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecules (Basel, Switzerland), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.7field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 1 country.

Kai ZhengCollege of Laboratory Medicine, Jilin Medical University, Jilin 132000, China.
Jiang HuCollege of Laboratory Medicine, Jilin Medical University, Jilin 132000, China.
Cheng HuCollege of Laboratory Medicine, Jilin Medical University, Jilin 132000, China.
Xueying LiuSchool of Medical Technology, Beihua University, Jilin 132000, China.
Yanyan WangSchool of Medical Technology, Beihua University, Jilin 132000, China.
Haojian HanCollege of Laboratory Medicine, Jilin Medical University, Jilin 132000, China.
Wenzhu XingSchool of Medical Technology, Beihua University, Jilin 132000, China.
Liu YangSchool of Medical Technology, Beihua University, Jilin 132000, China.
Junran ZhangZhiyuan College, Shanghai Jiao Tong University, Shanghai 200240, China.
Qiyuan HongCollege of Laboratory Medicine, Jilin Medical University, Jilin 132000, China.
Feng HaoCollege of Laboratory Medicine, Jilin Medical University, Jilin 132000, China.ORCID 0000-0002-2238-8654
Wenliang LiJilin Collaborative Innovation Center for Antibody Engineering, Jilin Medical University, Jilin 132000, China.
Jilin Medical University · CNBeihua University · CNShanghai Jiao Tong University · CN

Funding

Beihua University 2021006Beihua University 2022021Department of Education of Jilin Province JJKH20220462KJ, JJKH20220473SKJilin Medical University 2023jymkz002Jilin Medical University 2023zyc04Jilin Medical University JYBS2021013lkJilin Medical University S202213706026Jilin Province Science and Technology Department 20220101338JC
6 · The paper itself

Abstract

Transient receptor potential vanilloid 4 (TRPV4) is a widely expressed cation channel that plays an important role in many physiological and pathological processes. However, most TRPV4 drugs carry a risk of side effects. Moreover, existing screening methods are not suitable for the high-throughput screening (HTS) of drugs. In this study, a cell model and HTS method for targeting TRPV4 channel drugs were established based on a calcium-activated chloride channel protein 1 Anoctamin 1 (ANO1) and a double mutant (YFP-H148Q/I152L) of the yellow fluorescent protein (YFP). Patch-clamp experiments and fluorescence quenching kinetic experiments were used to verify that the model could sensitively detect changes in intracellular Ca

Indexed as

High-Throughput Screening AssaysTRPV Cation ChannelsAnoctamin-1CalciumAnoctamin-1CalciumTRPV Cation ChannelsANO1cell modeldrug screeninghigh-throughput screeningTRPV4YFP-H148Q/I152L

Identifiers

PMID38474548
PMCPMC10934975
OpenAlexW4392237825

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.