Evidence map›Paper›PMID 38473176›Full record

ArticleAnimals : an open access journal from MDPI2024

Deciphering Estrus Expression in Gilts: The Role of Alternative Polyadenylation and LincRNAs in Reproductive Transcriptomics.

Mingzheng Liu, Jiahao Chen, Chunlei Zhang, Shuhan Liu, Xiaohuan Chao, Huan Yang, Asim Muhammad, Bo Zhou, Weiping Ao, Allan P Schinckel

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Animals : an open access journal from MDPI, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.6field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Genome-wide association analysis highlights genomic regions and genes potentially associated with anestrus in crossbred gilts.Mammalian genome : official journal of the International Mammalian Genome Society · 2025
    Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 2 countries.

Mingzheng LiuCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Jiahao ChenCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Chunlei ZhangCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Shuhan LiuCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Xiaohuan ChaoCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Huan YangCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Asim MuhammadCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Bo ZhouCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.ORCID 0000-0003-2857-8536
Weiping AoCollege of Animal Science and Technology, Tarim University, Alar 843300, China.
Allan P SchinckelDepartment of Animal Sciences, Purdue University, West Lafayette, IN 47907-2054, USA.ORCID 0000-0002-8614-7198
Nanjing Agricultural University · CNPurdue University West Lafayette · USTarim University · CN

Funding

"JBGS" Project of Breeding Industry Revitalization in Jiangsu Province JBGS[2021]101Key Project of Tarim University TDZKZD202203National Natural Science Foundation of China NO. 32172786
6 · The paper itself

Abstract

The fertility rate and litter size of female pigs are critically affected by the expression of estrus. The objective of this study was to elucidate the regulatory mechanisms of estrus expression by analyzing the differential expression of genes and long intergenic non-coding RNAs (lincRNA), as well as the utilization of alternative polyadenylation (APA) sites, in the vulva and vagina during the estrus and diestrus stages of Large White and indigenous Chinese Mi gilts. Our study revealed that the number of differentially expressed genes (DEG) in the vulva was less than that in the vagina, and the DEGs in the vulva were enriched in pathways such as "neural" pathways and steroid hormone responses, including the "Calcium signaling pathway" and "Oxytocin signaling pathway". The DEGs in the vagina were enriched in the "Metabolic pathways" and "VEGF signaling pathway". Furthermore, 27 and 21 differentially expressed lincRNAs (DEL), whose target genes were enriched in the "Endocrine resistance" pathway, were identified in the vulva and vagina, respectively. Additionally, we observed that 63 and 618 transcripts of the 3'-untranslated region (3'-UTR) were lengthened during estrus in the vulva and vagina, respectively. Interestingly, the genes undergoing APA events in the vulva exhibited species-specific enrichment in neural or steroid-related pathways, whereas those in the vagina were enriched in apoptosis or autophagy-related pathways. Further bioinformatic analysis of these lengthened 3'-UTRs revealed the presence of multiple miRNAs binding sites and cytoplasmic polyadenylation element (CPE) regulatory aspects. In particular, we identified more than 10 CPEs in the validated lengthened 3'-UTRs of the

Indexed as

alternative polyadenylationestrusLncRNApigs

Identifiers

PMID38473176
PMCPMC10931002
OpenAlexW4392380568

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.