Evidence map›Paper›PMID 38472949›Full record

ArticleDiagnostics (Basel, Switzerland)2024

Assessing Different PCR Master Mixes for Ultrarapid DNA Amplification: Important Analytical Parameters.

Ivan Brukner, Miltiadis Paliouras, Mark Trifiro, Marc Bohbot, Daniel Shamir, Andrew G Kirk

Abstract read
In one paragraph

Article in Diagnostics (Basel, Switzerland), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Ivan BruknerLady Davis Institute for Medical Research, Jewish General Hospital, Montreal, QC H3T 1E2, Canada.ORCID 0000-0003-0225-9238
Miltiadis PaliourasDepartment of Medicine, McGill University, Montreal, QC H3G 2M1, Canada.ORCID 0000-0003-1321-4414
Mark TrifiroLady Davis Institute for Medical Research, Jewish General Hospital, Montreal, QC H3T 1E2, Canada.
Marc BohbotNexless Healthcare, 1315 Chem. Canora, Mont-Royal, Montreal, QC H3P 2J5, Canada.
Daniel ShamirNexless Healthcare, 1315 Chem. Canora, Mont-Royal, Montreal, QC H3P 2J5, Canada.
Andrew G KirkDepartment of Electrical and Computer Engineering, McGill University, Montreal, QC H3A 0E9, Canada.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The basic principles of ultrafast plasmonic PCR have been promulgated in the scientific and technological literature for over a decade. Yet, its everyday diagnostic utility remains unvalidated in pre-clinical and clinical settings. Although the impressive speed of plasmonic PCR reaction is well-documented, implementing this process into a device form compatible with routine diagnostic tasks has been challenging. Here, we show that combining careful system engineering and process control with innovative and specific PCR biochemistry makes it possible to routinely achieve a sensitive and robust "10 min" PCR assay in a compact and lightweight system. The critical analytical parameters of PCR reactions are discussed in the current instrument setting.

Indexed as

diagnosticsDNAmolecularPCRplasmonic PCRTaq polymerasetechnologyultrafast

Identifiers

PMID38472949
PMCPMC10931018

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.