Evidence map›Paper›PMID 38472563›Full record

ArticleJournal of assisted reproduction and genetics2024

Live birth outcomes following single-step blastocyst warming technique - optimizing efficiency without impacting live birth rates.

Victoria S Jiang, Panagiotis Cherouveim, Mackenzie N Naert, Irene Dimitriadis, Irene Souter, Charles L Bormann

Registry-linked trialAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of assisted reproduction and genetics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT06741748 (A Pragmatic, Multi-centre, Double-blinded, Two-arm Randomized Controlled Trial on a Direct Warming Frozen Embryo Transfer in Assisted Reproductive Technologies Treatment Outcome), which is not on this map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT06741748 nanot yet recruitingnot on this mapstarted 2025, after this paper: background citation

A Pragmatic, Multi-centre, Double-blinded, Two-arm Randomized Controlled Trial on a Direct Warming Frozen Embryo Transfer in Assisted Reproductive Technologies Treatment Outcome

TypeinterventionalSponsorChinese University of Hong KongRan2025 to 2027Enrolled578ConditionsFrozen Embryo Transfer (FET), Assisted Reproductive Techniques, In Vitro Fertilization (IVF), Cryopreservation of EmbryosArmsDirect Warming Method, Conventional Multi-step Thawing Method
3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

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  9. The new ice age: the promise and challenges of rapid oocyte warming protocols.Journal of assisted reproduction and genetics · 2024
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Victoria S JiangDivision of Reproductive Endocrinology and Infertility, Vincent Obstetrics and Gynecology, Massachusetts General Hospital, Harvard Medical School, 55 Fruit Street, Suite 10A, Boston, MA, 02114, USA.ORCID http://orcid.org/0000-0003-0625-1399
Panagiotis CherouveimDivision of Reproductive Endocrinology and Infertility, Vincent Obstetrics and Gynecology, Massachusetts General Hospital, Harvard Medical School, 55 Fruit Street, Suite 10A, Boston, MA, 02114, USA.ORCID http://orcid.org/0000-0003-0378-5336
Mackenzie N NaertVincent Obstetrics and Gynecology, Massachusetts General Hospital, Harvard Medical School, 32 Fruit Street, Suite 4F, Boston, MA, 02114, USA.ORCID http://orcid.org/0000-0002-2726-5864
Irene DimitriadisDivision of Reproductive Endocrinology and Infertility, Vincent Obstetrics and Gynecology, Massachusetts General Hospital, Harvard Medical School, 55 Fruit Street, Suite 10A, Boston, MA, 02114, USA.
Irene SouterDivision of Reproductive Endocrinology and Infertility, Vincent Obstetrics and Gynecology, Massachusetts General Hospital, Harvard Medical School, 55 Fruit Street, Suite 10A, Boston, MA, 02114, USA.ORCID http://orcid.org/0000-0003-3123-5438
Charles L BormannDivision of Reproductive Endocrinology and Infertility, Vincent Obstetrics and Gynecology, Massachusetts General Hospital, Harvard Medical School, 55 Fruit Street, Suite 10A, Boston, MA, 02114, USA. cbormann@mgb.org.ORCID http://orcid.org/0000-0003-3377-972X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeTo evaluate the impact of a single-step (SS) warming versus standard warming (SW) protocol on the survival/expansion of vitrified blastocysts and their clinical outcomes post-frozen embryo transfer (FET).

methodsRetrospective analysis was performed on 200 vitrified/warmed research blastocysts equally divided amongst two thawing protocols utilizing the Fujifilm Warming NX kits (Fujifilm, CA). SW utilized the standard 14-minute manufacturer's guidelines. SS protocol required only a one-minute immersion in thaw solution (TS) before the embryos were transferred to culture media. A time-interrupted study was performed evaluating 752 FETs (SW: 376 FETs, SS 376 FETs) between April 2021-December 2022 at a single academic fertility clinic in Boston, Massachusetts. Embryologic, clinical pregnancy, and live birth outcomes were assessed using generalized estimated equation (GEE) models, which accounted for potential confounders.

resultsThere was 100% survival for all blastocysts (n = 952 embryos) with no differences in blastocyst re-expansion regardless of PGT status. Adjusted analysis showed no differences in implantation, clinical pregnancy, spontaneous abortion, or biochemical pregnancy rate. A higher odds of multiple gestation [AdjOR(95%CI) 1.06 (1.01, 1.11), p = 0.019] were noted, even when adjusting for number of embryos transferred [AdjOR(95%CI) 1.05 (1.01, 1.10)]. Live birth outcomes showed no differences in live birth rates or birthweight at delivery.

conclusionsThe study found equivalent outcomes for SS and SW in all parameters except for a slight rise in the rate of multiple gestations. The results suggest that SS warming is an efficient, viable alternative to SW, reducing thaw times without adverse effects on live birth rates or neonatal birth weights.

Indexed as

Birth RateBlastocystCryopreservationEmbryo TransferLive BirthPregnancy RateVitrificationAdultEmbryo Culture TechniquesEmbryo ImplantationFemaleFertilization in VitroHumansPregnancyPregnancy OutcomeRetrospective StudiesBlastocyst warmingCryothaw protocolFrozen embryo transferLive birth rateSingle-step warming

Identifiers

PMID38472563
PMCPMC11143112

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Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.