Evidence map›Paper›PMID 38469310›Full record

ReviewFrontiers in immunology2024

Small extracellular vesicles purification and scale-up.

Xinya Zheng, Hongru Ai, Kewen Qian, Guangyao Li, Shuyi Zhang, Yitan Zou, Changhai Lei, Wenyan Fu, Shi Hu

Abstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Frontiers in immunology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed.

  1. Review
  2. Review
  3. Review
  4. Review
  5. Article
  6. Review
  7. Review
  8. Review
  9. Review
  10. Review
  11. Article
  12. Article
  13. Review
  14. Review
  15. Review
  16. Article
  17. Article
  18. Review
  19. Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Xinya ZhengDepartment of Biomedical Engineering, College of Basic Medical Sciences, Second Military Medical University, Shanghai, China.
Hongru AiDepartment of Biomedical Engineering, College of Basic Medical Sciences, Second Military Medical University, Shanghai, China.
Kewen QianDepartment of Biomedical Engineering, College of Basic Medical Sciences, Second Military Medical University, Shanghai, China.
Guangyao LiDepartment of Biophysics, College of Basic Medical Sciences, Second Military Medical University, Shanghai, China.
Shuyi ZhangDepartment of Biophysics, College of Basic Medical Sciences, Second Military Medical University, Shanghai, China.
Yitan ZouDepartment of Biomedical Engineering, College of Basic Medical Sciences, Second Military Medical University, Shanghai, China.
Changhai LeiDepartment of Biophysics, College of Basic Medical Sciences, Second Military Medical University, Shanghai, China.
Wenyan FuDepartment of Assisted Reproduction, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Shi HuDepartment of Biomedical Engineering, College of Basic Medical Sciences, Second Military Medical University, Shanghai, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Exosomes are small extracellular vesicles (sEVs) secreted by cells. With advances in the study of sEVs, they have shown great potential in the diagnosis and treatment of disease. However, sEV therapy usually requires a certain dose and purity of sEVs to achieve the therapeutic effect, but the existing sEV purification technology exists in the form of low yield, low purity, time-consuming, complex operation and many other problems, which greatly limits the application of sEVs. Therefore, how to obtain high-purity and high-quality sEVs quickly and efficiently, and make them realize large-scale production is a major problem in current sEV research. This paper discusses how to improve the purity and yield of sEVs from the whole production process of sEVs, including the upstream cell line selection and cell culture process, to the downstream isolation and purification, quality testing and the final storage technology.

Indexed as

ExosomesExtracellular VesiclesBiological TransportCell Culture TechniquesCell Lineindustrializationpurificationscale-upsmall extracellular vesiclestherapeutics

Identifiers

PMID38469310
PMCPMC10925713

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.