ArticleHeliyon2024
Comparison of amino acid release between enzymatic hydrolysis and acid autolysis of rainbow trout viscera.
Article in Heliyon, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 19 citations in OpenAlex.
- Circular Valorization of Yellowfin Tuna (Molecules (Basel, Switzerland) · 2026Article
- Valorization of fish processing by-products through combined enzymatic and microbial hydrolysis: nitrogen recovery and fertilizer efficiency in wheat.Frontiers in nutrition · 2026Article
- Preparation, characterization, antioxidant activity evaluation, and potential application of Peruvian squid liver protein hydrolysates.Food chemistry: X · 2025Article
- Optimization of Sequential Enzymatic Hydrolysis in Porcine Blood and the Influence on Peptide Profile and Bioactivity of Prepared Hydrolysates.International journal of molecular sciences · 2025Article
- Protein hydrolysates from fish wastes: nutritional characteristics and its inclusion in diets for Octopus maya.PloS one · 2025Article
- Enzymatic Hydrolysis Systems Enhance the Efficiency and Biological Properties of Hydrolysates from Frozen Fish Processing Co-Products.Marine drugs · 2024Article
- Optimization of the autolysis of rainbow trout viscera for amino acid release using response surface methodology.Open research Europe · 2024Article
- Protein hydrolysates in cell culture: Toward multi-omics characterization.Biotechnology progressReview
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fish protein hydrolysates were obtained from cultured rainbow trout (Oncorhynchus mykiss) viscera using commercial and endogenous enzymes. Two methods were employed for hydrolysis: acid autolysis (also known as silage) at room temperature for 10 days in acidic conditions, until total solubilisation, and enzymatic hydrolysis using Alcalase 2.4 LFG, Protana Prime, and the endogenous enzymes in the viscera. The effectiveness of both methods in releasing free amino acids (FAA) was assessed. After evaluating the results, the most effective enzymatic hydrolysis was optimized. The findings indicated that enzymatic hydrolysis with Alcalase, Protana Prime and endogenous enzymes combined for 7 h at a dose of 1% of protein, and a 7-day acid autolysis yielded the highest degree of hydrolysis (83.8% and 75.8%), a yield of FAA from viscera of 5.9% and 3.2%, and a yield of FAA from total protein of 71.3% and 52.5%, respectively. In conclusion, the use of commercial enzymes was more efficient in releasing amino acids, but endogenous enzymes showed a strong proteolytic capacity during acid autolysis, suggesting it also as a promising method to produce FAA-rich hydrolysates.
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