Evidence map›Paper›PMID 38468094›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

CRISPR-Cas9-Mediated Genome Editing in Paenibacillus polymyxa.

Giulia Ravagnan, Meliawati Meliawati, Jochen Schmid

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
4.5field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

  1. Exploring the Multifunctional Role ofInternational journal of molecular sciences · 2025
    Review
  2. From Biocontrol to Synthesis: Innovative Progress ofInternational journal of molecular sciences · 2025
    Review
  3. Advancing sustainable practices withAIMS microbiology · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Giulia RavagnanInstitute of Molecular Microbiology and Biotechnology, University of Münster, Münster, Germany.
Meliawati MeliawatiInstitute of Molecular Microbiology and Biotechnology, University of Münster, Münster, Germany.
Jochen SchmidInstitute of Molecular Microbiology and Biotechnology, University of Münster, Münster, Germany. jochen.schmid@uni-muenster.de.
University of Münster · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In recent years, the clustered regularly interspaced palindromic repeats-Cas (CRISPR-Cas) technology has become the method of choice for precision genome editing in many organisms due to its simplicity and efficacy. Multiplex genome editing, point mutations, and large genomic modifications are attractive features of the CRISPR-Cas9 system. These applications facilitate both the ease and velocity of genetic manipulations and the discovery of novel functions. In this protocol chapter, we describe the use of a CRISPR-Cas9 system for multiplex integration and deletion modifications, and deletions of large genomic regions by the use of a single guide RNA (sgRNA), and, finally, targeted point mutation modifications in Paenibacillus polymyxa.

Indexed as

Gene EditingPaenibacillus polymyxaCRISPR-Cas SystemsGenomeRNA, Guide, CRISPR-Cas SystemsRNA, Guide, CRISPR-Cas SystemsCas9CRISPRGenome engineeringMultiplexingPaenibacillus polymyxaPoint mutationsSingle sgRNA

Identifiers

PMID38468094
OpenAlexW4392667885

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.