Evidence map›Paper›PMID 38466541›Full record

ArticleVeterinary research communications2024

The first molecular characterization of Sarcocystis cameli in the Indian dromedary camels (Camelus dromedarius).

Shirish Dadarao Narnaware, Basanti Jyotsana, Rakesh Ranjan, Shyam Singh Dahiya, Ved Prakash

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Article in Veterinary research communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed
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1 citing paper in PubMed.

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5 · Who and what money

Authors and funding

5 authors.

Shirish Dadarao NarnawareICAR-Central Coastal Agricultural Research Institute, Old Goa, Goa, 403402, India. drshirish009@gmail.com.
Basanti JyotsanaICAR- National Research Centre On Camel, Bikaner, Rajasthan, 334001, India.
Rakesh RanjanICAR- National Research Centre On Camel, Bikaner, Rajasthan, 334001, India.
Shyam Singh DahiyaICAR- National Institute On Foot and Mouth Disease, Bhubaneswar, Odisha, 752050, India.
Ved PrakashICAR- National Research Centre On Camel, Bikaner, Rajasthan, 334001, India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In the present study, tissue samples (tongue, esophagus and heart) were investigated from dromedary camels of India for identification and characterization of Sarcocystis spp. using histopathology, PCR and gene sequencing. Genomic DNA extracted from these tissue samples was used for PCR amplification of the cytochrome c oxidase subunit I gene (cox1) of Sarcocystis spp. and the partial sequence of small subunit ribosomal RNA (18S rRNA) gene of the S. cameli. The PCR products were purified, sequenced and analyzed using bioinformatics tools. Based on phylogenetic analysis of the cox1 gene, the sequences of the present study clustered with those of S. cameli, hosted by dromedary camels of Iraq and a close association was observed with S. masoni hosted by dogs and alpacas of China. Until now, there are no 18S rRNA sequences of S. cameli available in GenBank and this is the first study recording 18S rRNA sequences of S. cameli which were grouped with S. masoni from alpaca of China and guanaco and llama of Argentina in phylogenetic analysis. These findings could be useful for further studies on the characterization through molecular epidemiology, genetic diversity and host specificity of S. cameli.

Indexed as

CamelusPhylogenyRNA, Ribosomal, 18SSarcocystisSarcocystosisAnimalsElectron Transport Complex IVIndiaElectron Transport Complex IVRNA, Ribosomal, 18S18S rRNACamelscox1IndiaMolecular characterizationSarcocystis cameli

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