ArticleeLife2024
A novel imaging method (FIM-ID) reveals that myofibrillogenesis plays a major role in the mechanically induced growth of skeletal muscle.
Article in eLife, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed, 6 citations in OpenAlex.
- The effects of chronic beetroot juice supplementation with resistance training on skeletal muscle mitochondrial and redox markers in middle-aged and older adults.European journal of applied physiology · 2026Article
- Research into resistance training response heterogeneity: a summary of the 2025 conference at the University of Jyväskylä.Journal of applied physiology (Bethesda, Md. : 1985) · 2026Review
- Mechanical Loading Induces the Radial Growth of Myofibrils and Myofibrillogenesis via an mTORC1-Dependent Mechanism.bioRxiv : the preprint server for biology · 2026Article
- Mechanical loading induces the longitudinal growth of muscle fibers via a rapamycin-insensitive mechanism.Science advances · 2026Article
- Load-induced human skeletal muscle hypertrophy: Mechanisms, myths, and misconceptions.Journal of sport and health science · 2025Review
- The Structural Adaptations That Mediate Mechanical Load-Induced Changes in Muscle Mass.Advances in experimental medicine and biology · 2025Review
- Review
- Optimizing Resistance Training for Sprint and Endurance Athletes: Balancing Positive and Negative Adaptations.Sports medicine (Auckland, N.Z.) · 2024Review
- The utility-and limitations-of the rodent synergist ablation model in examining mechanisms of skeletal muscle hypertrophy.American journal of physiology. Cell physiology · 2024Review
- Aberrant evoked calcium signaling and nAChR cluster morphology in aFrontiers in cell and developmental biology · 2024Article
- Effects of low-load resistance training with blood flow restriction on muscle fiber myofibrillar and extracellular area.Frontiers in physiology · 2024Article
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Authors and funding
11 authors at 1 institution in 2 countries.
Funding
Abstract
An increase in mechanical loading, such as that which occurs during resistance exercise, induces radial growth of muscle fibers (i.e. an increase in cross-sectional area). Muscle fibers are largely composed of myofibrils, but whether radial growth is mediated by an increase in the size of the myofibrils (i.e. myofibril hypertrophy) and/or the number of myofibrils (i.e. myofibrillogenesis) is not known. Electron microscopy (EM) can provide images with the level of resolution that is needed to address this question, but the acquisition and subsequent analysis of EM images is a time- and cost-intensive process. To overcome this, we developed a novel method for visualizing myofibrils with a standard fluorescence microscope (fluorescence imaging of myofibrils with image deconvolution [FIM-ID]). Images from FIM-ID have a high degree of resolution and contrast, and these properties enabled us to develop pipelines for automated measurements of myofibril size and number. After extensively validating the automated measurements, we used both mouse and human models of increased mechanical loading to discover that the radial growth of muscle fibers is largely mediated by myofibrillogenesis. Collectively, the outcomes of this study offer insight into a fundamentally important topic in the field of muscle growth and provide future investigators with a time- and cost-effective means to study it.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.