Evidence map›Paper›PMID 38465924›Full record

ArticleJournal of visualized experiments : JoVE2024

Optical Photothermal Infrared - Fluorescence In Situ Hybridization (OPTIR-FISH).

Zhongyue Guo, Yeran Bai, Fátima C Pereira, Ji-Xin Cheng

Abstract readVideo-Audio Media
In one paragraph

Article in Journal of visualized experiments : JoVE, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Zhongyue Guo *Department of Biomedical Engineering, Boston University.
Yeran Bai *Neuroscience Research Institute, Department of Molecular, Cellular, and Developmental Biology, University of California Santa Barbara; Photothermal Spectroscopy Corp.; yrbai@ucsb.edu.
Fátima C PereiraSchool of Biological Sciences, University of Southampton.
Ji-Xin ChengDepartment of Biomedical Engineering, Boston University; Department of Electrical & Computer Engineering, Photonics Center, Boston University; jxcheng@bu.edu.

Funding

Vibrational Spectroscopic Imaging to Unveil Hidden Signatures in Living SystemsR35GM136223 · NIGMS · BOSTON UNIVERSITY (CHARLES RIVER CAMPUS) · PI CHENG, JI-XIN · 2020 to 2025
$5.7M
Rapid AST through Metabolic Imaging at Single Cell LevelR01AI141439 · NIAID · BOSTON UNIVERSITY (CHARLES RIVER CAMPUS) · PI Ji-Xin Cheng · 2019 to 2026
$4.7M
NIAID NIH HHS R01 AI141439NIGMS NIH HHS R35 GM136223
6 · The paper itself

Abstract

Understanding the metabolic activities of individual cells within complex communities is critical for unraveling their role in human disease. Here, we present a comprehensive protocol for simultaneous cell identification and metabolic analysis with the OPTIR-FISH platform by combining rRNA-tagged FISH probes and isotope-labeled substrates. Fluorescence imaging provides cell identification by the specific binding of rRNA-tagged FISH probes, while OPTIR imaging provides metabolic activities within single cells by isotope-induced red shift on OPTIR spectra. Using bacteria cultured with

Indexed as

BacteriaRNA, RibosomalHumansIn Situ Hybridization, FluorescenceIsotopesOligonucleotide ProbesIsotopesOligonucleotide ProbesRNA, Ribosomal

Identifiers

PMID38465924
PMCPMC11797646

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.