Evidence map›Paper›PMID 38464329›Full record

ArticlebioRxiv : the preprint server for biology2024

Stiffness Measurement of Retinal Capillaries and Subendothelial Matrix using Atomic Force Microscopy.

Irene Santiago Tierno, Mahesh Agarwal, Nikolaos Matisioudis, Sathishkumar Chandrakumar, Kaustabh Ghosh

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Irene Santiago TiernoDepartment of Ophthalmology, University of California, Los Angeles, CA, USA.
Mahesh AgarwalDepartment of Ophthalmology, University of California, Los Angeles, CA, USA.ORCID 0000-0002-0529-0196
Nikolaos MatisioudisDoheny Eye Institute, Pasadena, CA, USA.
Sathishkumar ChandrakumarDepartment of Ophthalmology, University of California, Los Angeles, CA, USA.ORCID 0000-0003-2320-050X
Kaustabh GhoshDepartment of Ophthalmology, University of California, Los Angeles, CA, USA.ORCID 0000-0001-8185-9180
University of California, Los Angeles · USDoheny Eye Institute · US

Funding

Role of Retinal Capillary Stiffness in Diabetic RetinopathyR01EY028242 · NEI · UNIVERSITY OF CALIFORNIA RIVERSIDE · PI GHOSH, KAUSTABH · 2017 to 2021
$1.9M
NEI NIH HHS R01 EY028242
6 · The paper itself

Abstract

Retinal capillary degeneration is a clinical hallmark of the early stages of diabetic retinopathy (DR). Our recent studies have revealed that diabetes-induced increase in retinal capillary stiffness plays a crucial and previously unrecognized causal role in inflammation-mediated degeneration of retinal capillaries. Retinal capillary stiffening results from overexpression of lysyl oxidase, an enzyme that crosslinks and stiffens the subendothelial matrix. Since tackling DR at the early stage is expected to prevent or slow down DR progression and associated vision loss, subendothelial matrix and capillary stiffness represent relevant and novel therapeutic targets for early DR management. Further, direct measurement of retinal capillary stiffness can serve as a crucial preclinical validation step for the development of new imaging techniques for non-invasive assessment of retinal capillary stiffness in animal and human subjects. With this view in mind, we here provide a detailed protocol for the isolation and stiffness measurement of mouse retinal capillaries and retinal subendothelial matrix using atomic force microscopy.

Indexed as

Atomic force microscopyBasement membraneEndothelial cellsLysyl oxidaseMatrixRetinal vesselsStiffness

Identifiers

PMID38464329
PMCPMC10925338
OpenAlexW4392284564

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.