Evidence map›Paper›PMID 38464307›Full record

ArticlebioRxiv : the preprint server for biology2024

Hachiman is a genome integrity sensor.

Owen T Tuck, Benjamin A Adler, Emily G Armbruster, Arushi Lahiri, Jason J Hu, Julia Zhou, Joe Pogliano, Jennifer A Doudna

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 9 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 1 country.

Owen T TuckDepartment of Chemistry, University of California, Berkeley, Berkeley, CA USA.ORCID 0000-0002-0348-6910
Benjamin A AdlerInnovative Genomics Institute, University of California, Berkeley, Berkeley, CA USA.ORCID 0000-0002-7488-3040
Emily G ArmbrusterSchool of Biological Sciences, University of California San Diego, La Jolla, CA USA.ORCID 0000-0003-0377-1209
Arushi LahiriDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California USA.ORCID 0000-0001-6834-4692
Jason J HuDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California USA.ORCID 0000-0002-1103-2177
Julia ZhouDepartment of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California USA.ORCID 0000-0003-4084-0269
Joe PoglianoSchool of Biological Sciences, University of California San Diego, La Jolla, CA USA.
Jennifer A DoudnaDepartment of Chemistry, University of California, Berkeley, Berkeley, CA USA.ORCID 0000-0001-9161-999X
University of California, Berkeley · USQB3 · USUniversity of California San Diego · USInnovative Genomics Institute · US

Funding

RESEARCH PROJECT 2U19AI135990 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Nevan J Krogan · 2018 to 2026
$21.4M
Pathways in Biological Sciences Training ProgramT32GM133351 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Matthew Daugherty, Randolph Y. Hampton · 2020 to 2026
$9.9M
Molecular and cellular biology of the phage nucleus and spindleR01GM129245 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI POGLIANO, JOSEPH A · 2018 to 2025
$4.2M
Expanding CRISPR-Cas editing technology through exploration of novel Cas proteins and DNA repair systemsU01AI142817 · NIAID · UNIVERSITY OF CALIFORNIA BERKELEY · PI BANFIELD, JILLIAN, DOUDNA, JENNIFER A · 2018 to 2022
$2.0M
NIAID NIH HHS U01 AI142817NIAID NIH HHS U19 AI135990NIGMS NIH HHS R01 GM129245NIGMS NIH HHS T32 GM133351
6 · The paper itself

Abstract

Hachiman is a broad-spectrum antiphage defense system of unknown function. We show here that Hachiman comprises a heterodimeric nuclease-helicase complex, HamAB. HamA, previously a protein of unknown function, is the effector nuclease. HamB is the sensor helicase. HamB constrains HamA activity during surveillance of intact dsDNA. When the HamAB complex detects DNA damage, HamB helicase activity liberates HamA, unleashing nuclease activity. Hachiman activation degrades all DNA in the cell, creating 'phantom' cells devoid of both phage and host DNA. We demonstrate Hachiman activation in the absence of phage by treatment with DNA-damaging agents, suggesting that Hachiman responds to aberrant DNA states. Phylogenetic similarities between the Hachiman helicase and eukaryotic enzymes suggest this bacterial immune system has been repurposed for diverse functions across all domains of life.

Identifiers

PMID38464307
PMCPMC10925250
OpenAlexW4392386987

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.