Evidence map›Paper›PMID 38464277›Full record

ArticlebioRxiv : the preprint server for biology2024

Rapid

Md Mofizur Rahman, Lixue Wang, Md Motiar Rahman, Yundi Chen, Wenlong Zhang, Jing Wang, Luke P Lee, Yuan Wan

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 4 countries.

Md Mofizur RahmanThe Pq Laboratory of BiomeDx/Rx, Department of Biomedical Engineering, Binghamton University, Binghamton, NY, USA.
Lixue WangThe Pq Laboratory of BiomeDx/Rx, Department of Biomedical Engineering, Binghamton University, Binghamton, NY, USA.
Md Motiar RahmanDepartment of Chemistry, Binghamton University, Binghamton, NY, USA.
Yundi ChenThe Pq Laboratory of BiomeDx/Rx, Department of Biomedical Engineering, Binghamton University, Binghamton, NY, USA.
Wenlong ZhangTwist Bioscience Corporation, San Francisco, CA, USA.
Jing WangDepartment of Hematology, The Affiliated Drum Tower Hospital of Nanjing University Medical School, Nanjing, Jiangsu, China.
Luke P LeeHarvard Medical School, Harvard University; Department of Medicine, Brigham and Women's Hospital, Boston, MA, USA.
Yuan WanThe Pq Laboratory of BiomeDx/Rx, Department of Biomedical Engineering, Binghamton University, Binghamton, NY, USA.
Binghamton University · USBrigham and Women's Hospital · USNanjing Drum Tower Hospital · CNTwist Bioscience (United States) · US

Funding

Liquid biopsy of solitary pulmonary nodule with extracellular vesiclesR37CA255948 · NCI · STATE UNIVERSITY OF NY,BINGHAMTON · PI Yuan Wan · 2021 to 2026
$3.0M
Lipid nanoprobe integrated microdevice for extracellular vesicle isolation and duplex sequencing based mutation detection for non-invasive lung cancer diagnosisR01CA230339 · NCI · PENNSYLVANIA STATE UNIVERSITY, THE · PI ZHENG, SIYANG · 2018 to 2023
$1.9M
NCI NIH HHS R01 CA230339NCI NIH HHS R37 CA255948
6 · The paper itself

Abstract

A PCR- and sequencing-free mutation detection assay facilitates cancer diagnosis and reduces over-reliance on specialized equipment. This benefit was highlighted during the pandemic when high demand for viral nucleic acid testing often sidelined mutation analysis. This shift led to substantial challenges for patients on targeted therapy in tracking mutations. Here, we report a 30-minute DNA mutation detection technique using Cas12a-loaded liposomes in a microplate reader, a fundamental laboratory tool. CRISPR-Cas12a complex and fluorescence-quenching (FQ) probes are introduced into tumor-derived extracellular vesicles (EV) through membrane fusion. When CRISPR-RNA hybridizes with the DNA target, activated Cas12a can

Indexed as

CRISPR-Cas12DNA mutationEGFRLung cancertargeted therapy

Identifiers

PMID38464277
PMCPMC10925088
OpenAlexW4392293813

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.