Evidence map›Paper›PMID 38464062›Full record

ArticlebioRxiv : the preprint server for biology2024

Tissue-specific and endogenous protein labeling with split fluorescent proteins.

Gloria D Ligunas, German Paniagua, Jesselynn LaBelle, Adela Ramos-Martinez, Kyle Shen, Emma H Gerlt, Kaddy Aguilar, Alice Nguyen, Stefan C Materna, Stephanie Woo

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Gloria D LigunasDepartment of Molecular Cell Biology, University of California, Merced, CA USA.
German PaniaguaDepartment of Molecular Cell Biology, University of California, Merced, CA USA.
Jesselynn LaBelleDepartment of Molecular Cell Biology, University of California, Merced, CA USA.
Adela Ramos-MartinezDepartment of Molecular Cell Biology, University of California, Merced, CA USA.
Kyle ShenDepartment of Molecular Cell Biology, University of California, Merced, CA USA.
Emma H GerltDepartment of Molecular Cell Biology, University of California, Merced, CA USA.
Kaddy AguilarDepartment of Molecular Cell Biology, University of California, Merced, CA USA.
Alice NguyenDepartment of Molecular Cell Biology, University of California, Merced, CA USA.
Stefan C MaternaDepartment of Molecular Cell Biology, University of California, Merced, CA USA.
Stephanie WooDepartment of Molecular Cell Biology, University of California, Merced, CA USA.ORCID 0000-0002-1238-8167
University of California, Merced · US

Funding

G-RISE at UC MercedT32GM141862 · NIGMS · UNIVERSITY OF CALIFORNIA, MERCED · PI GOPINATHAN, AJAY, LIWANG, ANDY · 2021 to 2025
$2.7M
Tissue-specific labeling of endogenous proteins with split fluorescent proteinsR15HD102829 · NICHD · UNIVERSITY OF CALIFORNIA, MERCED · PI WOO, STEPHANIE · 2020 to 2022
$578k
NICHD NIH HHS R15 HD102829NIGMS NIH HHS T32 GM141862
6 · The paper itself

Abstract

The ability to label proteins by fusion with genetically encoded fluorescent proteins is a powerful tool for understanding dynamic biological processes. However, current approaches for expressing fluorescent protein fusions possess drawbacks, especially at the whole organism level. Expression by transgenesis risks potential overexpression artifacts while fluorescent protein insertion at endogenous loci is technically difficult and, more importantly, does not allow for tissue-specific study of broadly expressed proteins. To overcome these limitations, we have adopted the split fluorescent protein system mNeonGreen2

Indexed as

CRISPR-Casprotein taggingsplit fluorescent proteinZebrafish

Identifiers

PMID38464062
PMCPMC10925240
OpenAlexW4392291963

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.