Evidence map›Paper›PMID 38464036›Full record

ArticlebioRxiv : the preprint server for biology2024

Engineering an Escherichia coli strain for production of long single-stranded DNA.

Konlin Shen, Jake J Flood, Zhihuizi Zhang, Alvin Ha, Brian R Shy, John E Dueber, Shawn M Douglas

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors.

Konlin ShenDepartment of Cellular and Molecular Pharmacology, University of California, San Francisco, CA, USA.ORCID 0000-0003-1184-7605
Jake J FloodDepartment of Bioengineering, University of California, Berkeley, CA, USA.ORCID 0000-0003-0041-5794
Zhihuizi ZhangDepartment of Cellular and Molecular Pharmacology, University of California, San Francisco, CA, USA.ORCID 0009-0003-7974-0318
Alvin HaDepartment of Laboratory Medicine, University of California San Francisco, San Francisco, CA, USA.ORCID 0000-0002-7092-9072
Brian R ShyDepartment of Laboratory Medicine, University of California San Francisco, San Francisco, CA, USA.ORCID 0000-0001-9569-3708
John E DueberDepartment of Bioengineering, University of California, Berkeley, CA, USA.ORCID 0000-0001-8404-7153
Shawn M DouglasDepartment of Cellular and Molecular Pharmacology, University of California, San Francisco, CA, USA.ORCID 0000-0001-5398-9041

Funding

DNA Nanostructures for High-Throughput Cryo-EM Studies of Small MacromoleculesR35GM125027 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Shawn M Douglas · 2018 to 2026
$3.8M
Defining the role of ligand spatial organization in T cell signaling with DNA origamiF32GM147967 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI SHEN, KONLIN · 2023 to 2023
$70k
NIGMS NIH HHS F32 GM147967NIGMS NIH HHS R35 GM125027
6 · The paper itself

Abstract

Long single-stranded DNA (ssDNA) is a versatile molecular reagent with applications including RNA-guided genome engineering and DNA nanotechnology, yet its production is typically resource-intensive. We introduce a novel method utilizing an engineered

Identifiers

PMID38464036
PMCPMC10925301

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.