Evidence map›Paper›PMID 38463262›Full record

ArticleACS omega2024

Bipolar Clark-Type Oxygen Electrode Arrays for Imaging and Multiplexed Measurements of the Respiratory Activity of Cells.

Yusuke Shirato, An-Ju Hsueh, Nurul Asyikeen Ab Mutalib, Yi Deng, Ryohei Suematsu, Azusa Kato, Bradley M Kearney, Manabu Kinoshita, Hiroaki Suzuki

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS omega, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Yusuke ShiratoGraduate School of Science and Technology, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8573, Japan.ORCID https://orcid.org/0000-0003-0140-6574
An-Ju HsuehGraduate School of Science and Technology, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8573, Japan.ORCID https://orcid.org/0000-0001-7992-1600
Nurul Asyikeen Ab MutalibGraduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8573, Japan.ORCID https://orcid.org/0000-0002-9669-8997
Yi DengGraduate School of Science and Technology, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8573, Japan.ORCID https://orcid.org/0000-0001-9920-7789
Ryohei SuematsuDepartment of Immunology and Microbiology, National Defense Medical College, Namiki 3-2, Tokorozawa 359-8513, Japan.ORCID https://orcid.org/0000-0002-5038-874X
Azusa KatoDepartment of Immunology and Microbiology, National Defense Medical College, Namiki 3-2, Tokorozawa 359-8513, Japan.ORCID https://orcid.org/0000-0001-9976-2248
Bradley M KearneyDepartment of Immunology and Microbiology, National Defense Medical College, Namiki 3-2, Tokorozawa 359-8513, Japan.ORCID https://orcid.org/0000-0003-3037-0945
Manabu KinoshitaDepartment of Immunology and Microbiology, National Defense Medical College, Namiki 3-2, Tokorozawa 359-8513, Japan.ORCID https://orcid.org/0000-0002-2750-3084
Hiroaki SuzukiGraduate School of Science and Technology, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8573, Japan.ORCID https://orcid.org/0000-0002-3535-703X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Various miniature Clark-type oxygen electrodes (COEs), which are typically used to measure dissolved oxygen (DO) concentration in cellular respiration, have been developed since the 1980s. Arrays with individually addressable electrodes that constitute the sensor were used for various applications. However, the large number of leads and contact pads required for connecting the electrodes and the external instrument complicate the electrode layout and make the operation of integrated COE arrays challenging. Here, we fabricated closed bipolar electrochemical systems comprising 6 × 8 and 4 × 4 arrays of COEs for imaging and multiplexed detection. The cathodic compartment was sealed with a hydrophobic oxygen-permeable membrane to separate the internal electrolyte solution from the sample solutions. Using the bipolar Clark-type oxygen electrode (BCOE) arrays and electrochemiluminescence (ECL), we measured the DO concentration at each cathode. The results revealed that the ECL intensity changed linearly with the DO concentration. In addition, we used ECL imaging to investigate the respiratory activity of

Identifiers

PMID38463262
PMCPMC10918805

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.