Evidence map›Paper›PMID 38457625›Full record

ReviewChembiochem : a European journal of chemical biology2024

Measuring Ligand-bound Protein Complexes with Proximity Labeling: A Practical Guide.

Pavitra Goyal, Andrew J Tao, Elizabeth J Mumby, Justin G English, Fleur M Ferguson

Abstract readReview
In one paragraph

Review in Chembiochem : a European journal of chemical biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Pavitra GoyalDepartment of Chemistry and Biochemistry, University of California San Diego, La Jolla, CA 92093.ORCID 0009-0005-9219-2736
Andrew J TaoDepartment of Chemistry and Biochemistry, University of California San Diego, La Jolla, CA 92093.ORCID 0000-0003-1576-986X
Elizabeth J MumbyDepartment of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT 84112.ORCID 0000-0002-0395-4307
Justin G EnglishDepartment of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT 84112.ORCID 0000-0002-1161-8928
Fleur M FergusonDepartment of Chemistry and Biochemistry, University of California San Diego, La Jolla, CA 92093.ORCID 0000-0003-4091-7617

Funding

VIRAL MALIGNANCYP30CA023100 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Jesse Nodora · 1985 to 2026
$124.9M
Contemporary Approaches to Cancer Cell Signaling and CommunicationT32CA009523 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI DONOGHUE, DANIEL J, YANG, JING · 1985 to 2025
$12.1M
Chemical Control of Misfolded Protein FateDP2NS132610 · NINDS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI FERGUSON, FLEUR MARCIA · 2022 to 2025
$2.4M
Directed Evolution of Cell-Type Specific On-Demand Signaling Control SystemsDP2GM146247 · NIGMS · UNIVERSITY OF UTAH · PI ENGLISH, JUSTIN G. · 2021 to 2024
$2.3M
American Cancer Society IRG IRG-19-230-48-IRGNCI NIH HHS P30 CA023100NCI NIH HHS T32 CA009523NIGMS NIH HHS DP2 GM146247NIH HHS DP2GM146247NIH HHS DP2NS132610NINDS NIH HHS DP2 NS132610UC San Diego Moores Cancer Center, Specialized Cancer Center Support NIH/NCI P30CA023100UCSD
6 · The paper itself

Abstract

Identifying the drug-target interactome of small molecule therapeutics is essential for understanding the full pharmacological effects of a compound. These therapies often induce changes within the cellular proteome, leading to unexpected consequences such as changes in the targets complexation state or off-target interactions between the compound and additional proteins. Currently, unbiased target-ID approaches are being used to embark on this task. Here we provide an overview of the strengths and limitations of these methods, and a practical step-by-step protocol for using the BioTAC system to assist with drug target and interactome ID.

Indexed as

ProteinsHumansLigandsProtein BindingLigandsProteins

Identifiers

PMID38457625
PMCPMC11663430

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.