Evidence map›Paper›PMID 38453771›Full record

ArticleReproductive sciences (Thousand Oaks, Calif.)2024

Amniotic Fluid Proteomics Analysis and In Vitro Validation to Identify Potential Biomarkers of Preterm Birth.

Siguo Chen, Weizhou Wang, Guanghong Yan, Mengmei Liu, Min Li, Ping Chen, Qingyan Ma, Jinman Zhang, Ying Tang, Linglin Zhou and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Reproductive sciences (Thousand Oaks, Calif.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Siguo Chen *Yunnan Provincial Key Laboratory of Public Health and Biosafety & School of Public Health, Kunming Medical University, Kunming, Yunnan, 650000, China.ORCID 0009-0002-0405-8915
Weizhou Wang *Department of Orthopedics, The First Affiliated Hospital of Kunming Medical University, Kunming, 650032, Yunnan, China.
Guanghong YanYunnan Provincial Key Laboratory of Public Health and Biosafety & School of Public Health, Kunming Medical University, Kunming, Yunnan, 650000, China.
Mengmei LiuYunnan Provincial Key Laboratory of Public Health and Biosafety & School of Public Health, Kunming Medical University, Kunming, Yunnan, 650000, China.
Min LiYunnan Provincial Key Laboratory of Public Health and Biosafety & School of Public Health, Kunming Medical University, Kunming, Yunnan, 650000, China.
Ping ChenYunnan Provincial Key Laboratory of Public Health and Biosafety & School of Public Health, Kunming Medical University, Kunming, Yunnan, 650000, China.
Qingyan MaYunnan Provincial Key Laboratory of Public Health and Biosafety & School of Public Health, Kunming Medical University, Kunming, Yunnan, 650000, China.
Jinman ZhangDepartment of Medical Genetics, First People's Hospital of Yunnan Province, Kunming, 650034, Yunnan, China.
Ying TangThe Electron Microscopy Laboratory, Science and Technology Achievement Incubation Center, Kunming Medical University, Kunming, 650000, Yunnan, China.
Linglin ZhouDepartment of Obstetrics, Gejiu City People's Hospital, Gejiu, 661000, Yunnan, China.
Dingyun YouYunnan Provincial Key Laboratory of Public Health and Biosafety & School of Public Health, Kunming Medical University, Kunming, Yunnan, 650000, China. youdingyun@qq.com.

Funding

Applied Basic Research Foundation of Yunnan Province 202001AW070021Basic Research of Yunnan Province Joint Special Project of Kunming Medical University 202101AY070001-195Basic Research of Yunnan Province - Key Project 202101AS070040Innovative Research Group Project of the National Natural Science Foundation of China 82073569National Natural Science Foundation of China 81960592National Natural Science Foundation of China 82260645Special Project for Selection of High-level Scientific and Technological Talents and Innovation Team-Reserve Talent Program for Young and Middle-aged Academic and Technical Leaders 202005AC160023
6 · The paper itself

Abstract

This study aimed to investigate the regulation of amniotic fibroblast (AFC) function by vitamin K-dependent protein Z (PROZ) during preterm birth (PTB) and its potential role in adverse pregnancy outcomes. Proteomic samples were collected from amniotic fluid in the second trimester, and AFC were isolated from the amniotic membrane and cultured in vitro. The expression of extracellular and intracellular PROZ in AFC was modulated, and their biological properties and functions were evaluated. Clinical analysis revealed a significant upregulation of PROZ expression in amniotic fluid from preterm pregnant women. In vitro experiments demonstrated that PROZ stimulated AFC migration, enhanced their proliferative capacity, and reduced collagen secretion. Overexpression of PROZ further enhanced cell migration and proliferation, while knockdown of PROZ had the opposite effect. PROZ plays a crucial role in promoting the proliferation and migration of amniotic membrane fibroblasts. Increased PROZ expression in amniotic fluid is associated with the occurrence of PTB. These findings shed light on the potential involvement of PROZ in adverse pregnancy outcomes and provide a basis for further research on its regulatory mechanisms during PTB.

Indexed as

Amniotic FluidBiomarkersCell MovementCell ProliferationPremature BirthProteomicsAdultAntimicrobial Cationic PeptidesBlood ProteinsCells, CulturedFemaleFibroblastsHumansPregnancyAntimicrobial Cationic PeptidesAZU1 protein, humanBiomarkersBlood ProteinsAmniotic fibroblastPremature birthProteomicsVitamin K–dependent protein Z

Identifiers

PMID38453771
PMCPMC11217130

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.