Evidence map›Paper›PMID 38450861›Full record

ArticleInsect molecular biology2024

Alpha-mannosidase-2 modulates arbovirus infection in a pathogen- and Wolbachia-specific manner in Aedes aegypti mosquitoes.

Nadya Urakova, Renuka E Joseph, Allyn Huntsinger, Vanessa M Macias, Matthew J Jones, Leah T Sigle, Ming Li, Omar S Akbari, Zhiyong Xi, Konstantinos Lymperopoulos and 3 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Insect molecular biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 4 institutions in 1 country.

Nadya UrakovaDepartment of Entomology, The Pennsylvania State University, University Park, Pennsylvania, USA.
Renuka E JosephDepartment of Entomology, The Pennsylvania State University, University Park, Pennsylvania, USA.
Allyn HuntsingerDepartment of Entomology, The Pennsylvania State University, University Park, Pennsylvania, USA.
Vanessa M MaciasDepartment of Entomology, The Pennsylvania State University, University Park, Pennsylvania, USA.
Matthew J JonesDepartment of Entomology, The Pennsylvania State University, University Park, Pennsylvania, USA.
Leah T SigleDepartment of Entomology, The Pennsylvania State University, University Park, Pennsylvania, USA.
Ming LiDivision of Biological Sciences, Section of Cell and Developmental Biology, University of California, San Diego, La Jolla, California, USA.
Omar S AkbariDivision of Biological Sciences, Section of Cell and Developmental Biology, University of California, San Diego, La Jolla, California, USA.
Zhiyong XiDepartment of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan, USA.
Konstantinos LymperopoulosPebble Labs, Little Fly Division, Los Alamos, New Mexico, USA.
Richard T SayrePebble Labs, Little Fly Division, Los Alamos, New Mexico, USA.
Elizabeth A McGrawDepartment of Entomology, The Pennsylvania State University, University Park, Pennsylvania, USA.
Jason L RasgonDepartment of Entomology, The Pennsylvania State University, University Park, Pennsylvania, USA.ORCID 0000-0002-4050-8429
Pennsylvania State University · USNew Mexico Consortium · USUniversity of California San Diego · USMichigan State University · US

Funding

Hologenomic basis of WNV vector competence in Culex tarsalisR01AI150251 · NIAID · PENNSYLVANIA STATE UNIVERSITY, THE · PI RASGON, JASON L · 2019 to 2024
$2.7M
Development of synthetic viral circuitry to regulate viruses in mosquito disease vectorsDP2AI152071 · NIAID · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI AKBARI, OMAR SULTAN · 2019 to 2019
$2.4M
Precision guided SIT for the control of vector-borne diseaseR01AI151004 · NIAID · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI AKBARI, OMAR SULTAN · 2020 to 2024
$2.3M
Wolbachia-induced enhancement of human arboviral pathogensR01AI116636 · NIAID · PENNSYLVANIA STATE UNIVERSITY, THE · PI RASGON, JASON L · 2016 to 2020
$1.9M
Experimental evolution of Wolbachia-mediated dengue virus blocking in the mosquito, Aedes aegyptiR01AI143758 · NIAID · PENNSYLVANIA STATE UNIVERSITY, THE · PI CHENOWETH, STEPHEN, MCGRAW, ELIZABETH ANN · 2019 to 2022
$1.5M
Development of precision genome editing tools in Ae. albopictus for functional genetics and mosquito control technologiesR21AI156078 · NIAID · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI AKBARI, OMAR SULTAN · 2021 to 2022
$434k
Defense Advanced Research Projects Agency HR0011-17-2-0047Dorothy Foehr Huck and J. Lloyd Huck EndowmentNational Science Foundation 1645331NIAID NIH HHS DP2 AI152071NIAID NIH HHS R01 AI116636NIAID NIH HHS R01 AI143758NIAID NIH HHS R01 AI150251NIAID NIH HHS R01 AI151004NIAID NIH HHS R21 AI156078NIH HHS DP2AI152071NIH HHS R01AI116636NIH HHS R01AI143758NIH HHS R01AI150251NIH HHS R01AI151004NIH HHS R21AI156078Pebble LabsUnited States Agency for International Development AID-OAA-F-16-00082
6 · The paper itself

Abstract

Multiple Wolbachia strains can block pathogen infection, replication and/or transmission in Aedes aegypti mosquitoes under both laboratory and field conditions. However, Wolbachia effects on pathogens can be highly variable across systems and the factors governing this variability are not well understood. It is increasingly clear that the mosquito host is not a passive player in which Wolbachia governs pathogen transmission phenotypes; rather, the genetics of the host can significantly modulate Wolbachia-mediated pathogen blocking. Specifically, previous work linked variation in Wolbachia pathogen blocking to polymorphisms in the mosquito alpha-mannosidase-2 (αMan2) gene. Here we use CRISPR-Cas9 mutagenesis to functionally test this association. We developed αMan2 knockouts and examined effects on both Wolbachia and virus levels, using dengue virus (DENV; Flaviviridae) and Mayaro virus (MAYV; Togaviridae). Wolbachia titres were significantly elevated in αMan2 knockout (KO) mosquitoes, but there were complex interactions with virus infection and replication. In Wolbachia-uninfected mosquitoes, the αMan2 KO mutation was associated with decreased DENV titres, but in a Wolbachia-infected background, the αMan2 KO mutation significantly increased virus titres. In contrast, the αMan2 KO mutation significantly increased MAYV replication in Wolbachia-uninfected mosquitoes and did not affect Wolbachia-mediated virus blocking. These results demonstrate that αMan2 modulates arbovirus infection in A. aegypti mosquitoes in a pathogen- and Wolbachia-specific manner, and that Wolbachia-mediated pathogen blocking is a complex phenotype dependent on the mosquito host genotype and the pathogen. These results have a significant impact for the design and use of Wolbachia-based strategies to control vector-borne pathogens.

Indexed as

Aedesalpha-MannosidaseWolbachiaAnimalsArbovirusesArbovirus InfectionsCRISPR-Cas SystemsDengue VirusFemaleInsect ProteinsMosquito Vectorsalpha-MannosidaseInsect Proteinsalpha‐mannosidase‐2CRISPRdengue virusMayaro virusWolbachia

Identifiers

PMID38450861
PMCPMC11233229
OpenAlexW4392560842

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.