ArticleScience advances2024
Single-cell nanobiopsy enables multigenerational longitudinal transcriptomics of cancer cells.
Article in Science advances, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Review
- Perspective on Scanning Electrochemical Probe Microscopy in Single-Cell Imaging.Chemical & biomedical imaging · 2026Review
- Analytical Methods and Application of Single-Cell and Single-Nucleus Transcriptomics in the Study of Ischemic Stroke.Biomolecules · 2026Review
- Opto-Electrochemical Probes for In Vitro/In Vivo Analysis: Principles, Designs, and Applications.Biosensors · 2026Review
- Applications of Nanopipettes in Scanning Ion Conductance Microscopy for High-Spatial-Resolution Topographic Imaging and Sensing in Single Cells.ACS measurement science au · 2026Review
- A new paradigm for tumour profiling: Spatiotemporal omics in living tissue.Clinical and translational medicine · 2025Article
- Delivery of Biomolecules into Individual Cells and Subcellular Compartments by Localized Electroporation via Nanopipette.ACS nanoscience Au · 2025Article
- Nanoneedles enable spatiotemporal lipidomics of living tissues.Nature nanotechnology · 2025Article
- Engineering next-generation microfluidic technologies for single-cell phenomics.Nature genetics · 2025Review
- Spatial and Temporal Single-Cell Profiling of RNA Compartmentalization in Neurons with Nanotweezers.ACS nano · 2025Article
- Nanopore approaches for single-molecule temporal omics: promises and challenges.Nature methods · 2025Review
- A subcellular sampling instrument allows spatial resolution of amyloid deposit-derived organelle-specific effects in microglia.Communications biology · 2025Article
- Nanopipettes as a Potential Diagnostic Tool for Selective Nanopore Detection of Biomolecules.Biosensors · 2024Review
- Nanobiopsy investigation of the subcellular mtDNA heteroplasmy in human tissues.Scientific reports · 2024Article
- Single molecule delivery into living cells.Nature communications · 2024Article
- Recent Developments in Single-Entity Electrochemistry.Analytical chemistry · 2024Review
Corrections and comments
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Authors and funding
12 authors.
Funding
Abstract
Single-cell RNA sequencing has revolutionized our understanding of cellular heterogeneity, but routine methods require cell lysis and fail to probe the dynamic trajectories responsible for cellular state transitions, which can only be inferred. Here, we present a nanobiopsy platform that enables the injection of exogenous molecules and multigenerational longitudinal cytoplasmic sampling from a single cell and its progeny. The technique is based on scanning ion conductance microscopy (SICM) and, as a proof of concept, was applied to longitudinally profile the transcriptome of single glioblastoma (GBM) brain tumor cells in vitro over 72 hours. The GBM cells were biopsied before and after exposure to chemotherapy and radiotherapy, and our results suggest that treatment either induces or selects for more transcriptionally stable cells. We envision the nanobiopsy will contribute to transforming standard single-cell transcriptomics from a static analysis into a dynamic assay.
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