Evidence map›Paper›PMID 38444827›Full record

ArticleFrontiers in cell and developmental biology2024

A versatile kinase mobility shift assay (KiMSA) for PKA analysis and cyclic AMP detection in sperm physiology (and beyond).

Analia G Novero, Catalina Curcio, Tomás J Steeman, Andres Binolfi, Diego Krapf, Mariano G Buffone, Dario Krapf, Cintia Stival

Open access · goldAbstract read
In one paragraph

Article in Frontiers in cell and developmental biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.9field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 2 countries.

Analia G NoveroCell Signal Transduction Networks, Instituto de Biología Molecular y Celular de Rosario (IBR) (CONICET-UNR), Rosario, Argentina.
Catalina CurcioCell Signal Transduction Networks, Instituto de Biología Molecular y Celular de Rosario (IBR) (CONICET-UNR), Rosario, Argentina.
Tomás J SteemanCell Signal Transduction Networks, Instituto de Biología Molecular y Celular de Rosario (IBR) (CONICET-UNR), Rosario, Argentina.
Andres BinolfiCellular-Structural Biology Lab, IBR (CONICET-UNR), Rosario, Argentina.
Diego KrapfDepartment of Electrical and Computer Engineering, Colorado State University, Fort Collins, CO, United States.
Mariano G BuffoneInstituto de Biología y Medicina Experimental (IBYME-CONICET), Ciudad Autónoma de Buenos Aires, Argentina.
Dario Krapf *Cell Signal Transduction Networks, Instituto de Biología Molecular y Celular de Rosario (IBR) (CONICET-UNR), Rosario, Argentina.
Cintia Stival *Cell Signal Transduction Networks, Instituto de Biología Molecular y Celular de Rosario (IBR) (CONICET-UNR), Rosario, Argentina.
Consejo Nacional de Investigaciones Científicas y Técnicas · AR

Funding

Regulation of Soluble Adenylate Cyclase During Mammalian Sperm CapacitationR01HD106968 · NICHD · COLORADO STATE UNIVERSITY · PI Diego Krapf · 2022 to 2026
$1.5M
NICHD NIH HHS R01 HD106968
6 · The paper itself

Abstract

The cAMP-dependent protein kinase (PKA) is one of the most extensively distributed kinases among intracellular signal cascades, with a pivotal role in the regulation of various processes, including the capacitation of sperm cells. Traditional assessments of PKA activity relies on the utilization of [γ-

Indexed as

cAMPfertilitykemptide kinase activitykinase assaynon-radioactive assayphosphorylationprotein kinase A (PKA)sperm capacitation

Identifiers

PMID38444827
PMCPMC10912184
OpenAlexW4391970136

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.