ArticleScientific reports2024
Extraction and separation of astaxanthin with the help of pre-treatment of Haematococcus pluvialis microalgae biomass using aqueous two-phase systems based on deep eutectic solvents.
Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 39 citations in OpenAlex.
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- Mild One-Step Protein Recovery from Microalgae Cultivated in Swine Wastewater Using Natural Deep Eutectic Solvent-Based Aqueous Biphasic Systems.Molecules (Basel, Switzerland) · 2026Article
- Resolving the "Thick-Wall Challenge" inMicroorganisms · 2026Review
- Current advancements and future prospects in the biorefinery and hyper-production of astaxanthin fromBioImpacts : BI · 2026Review
- Deep eutectic solvents as an emerging platform for microbial carotenoids extraction: State of the art, prospects, and challenges.Food chemistry: X · 2026Review
- Mechanism and Potential of Aqueous Enzymatic Extraction for Constructing Green Production System for Lipids and Proteins.Foods (Basel, Switzerland) · 2025Review
- Influence of mixing time and agitation speed on partitioning and mass transfer coefficient of cephalexin in aqueous two-phase systems.Scientific reports · 2025Article
- Marine-Derived Astaxanthin: Molecular Mechanisms, Biomedical Applications, and Roles in Stem Cell Biology.Marine drugs · 2025Review
- Separation of heavy metals using aqueous two-phase systems based on choline chloride/urea and betaine/urea deep eutectic solvents.Scientific reports · 2025Article
- Unveiling astaxanthin: biotechnological advances, delivery systems and versatile applications in nutraceuticals and cosmetics.Archives of microbiology · 2025Review
- Fine-Tuning Porous Structure of Zirconium-Based Metal-Organic Frameworks for Efficient Separation and Purification of Astaxanthin by Defect Engineering.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Physicochemical Characterization and Thermodynamic Analysis of Avocado Oil Enhanced withFoods (Basel, Switzerland) · 2024Article
- Recent Advances in the Therapeutic Potential of Carotenoids in Preventing and Managing Metabolic Disorders.Plants (Basel, Switzerland) · 2024Review
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3 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The microalgae Haematococcus pluvialis are the main source of the natural antioxidant astaxanthin. However, the effective extraction of astaxanthin from these microalgae remains a significant challenge due to the rigid, non-hydrolyzable cell walls. Energy savings and high-efficiency cell disruption are essential steps in the recovery of the antioxidant astaxanthin from the cysts of H. pluvialis. In the present study, H. pluvialis microalgae were first cultured in Bold's Basal medium under certain conditions to reach the maximum biomass concentration, and then light shock was applied for astaxanthin accumulation. The cells were initially green and oval, with two flagella. As the induction time increases, the motile cells lose their flagellum and become red cysts with thick cell walls. Pre-treatment of aqueous two-phase systems based on deep eutectic solvents was used to decompose the cell wall. These systems included dipotassium hydrogen phosphate salt, water, and two types of deep eutectic solvents (choline chloride-urea and choline chloride-glucose). The results of pre-treatment of Haematococcus cells by the studied systems showed that intact, healthy cysts were significantly ruptured, disrupted, and facilitated the release of cytoplasmic components, thus facilitating the subsequent separation of astaxanthin by liquid-liquid extraction. The system containing the deep eutectic solvent of choline chloride-urea was the most effective system for cell wall degradation, which resulted in the highest ability to extract astaxanthin. More than 99% of astaxanthin was extracted from Haematococcus under mild conditions (35% deep eutectic solvent, 30% dipotassium hydrogen phosphate at 50 °C, pH = 7.5, followed by liquid-liquid extraction at 25 °C). The present study shows that the pre-treatment of two-phase systems based on deep eutectic solvent and, thus, liquid-liquid extraction is an efficient and environmentally friendly process to improve astaxanthin from the microalgae H. pluvialis.
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