ArticleThe Plant cell2024
OPEN STOMATA 1 phosphorylates CYCLIC NUCLEOTIDE-GATED CHANNELs to trigger Ca2+ signaling for abscisic acid-induced stomatal closure in Arabidopsis.
Article in The Plant cell, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 46 citations in OpenAlex.
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- Synthetic ion channel inhibitors enhance plant drought tolerance.Nature communications · 2026Article
- Nitric Oxide Enhances Drought Tolerance inPlants (Basel, Switzerland) · 2026Article
- Melatonin enhances the cold tolerance of tomatoes by promoting ABA accumulation.Plant cell reports · 2026Article
- Osmotic signaling governs sunscreen biosynthesis to safeguard desert cyanobacteria against desiccation.mLife · 2026Article
- TaCNGC-2A suppresses seed dormancy and activates pre-harvest sprouting through modulating calcium and hormonal signaling pathways.Nature communications · 2026Article
- A BRI1-CNGC12 phosphorylation module links hormone signaling to manganese homeostasis in plants.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Abscisic Acid and Calcium Signals Convergently Regulate Sugar Accumulation by Orchestrating the SRK2A/CIPK6-ABI5-TST2 Module in Citrus.Plant biotechnology journal · 2026Article
- A drought stress-responsive metabolite malate modulates stomatal responses through G-protein-dependent pathway in grapevine and Arabidopsis.Molecular horticulture · 2026Article
- MAP4K1 and MAP4K2 regulate ABA-induced and CaScience advances · 2025Article
- Roles of ROS and NO in Plant Responses to Individual and Combined Salt Stress and Waterlogging.Antioxidants (Basel, Switzerland) · 2025Review
- Regulation of vacuole fusion in stomata by dephosphorylation of the HOPS subunit VPS39.The Plant journal : for cell and molecular biology · 2025Article
- OPEN STOMATA 1 activates SLAC1 anion channel primarily through CPK15 in ABA-induced stomatal closure inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Maintaining basal B-RAF kinase activity for abscisic acid signaling via reciprocal phosphoregulation of a single serine residue.Journal of integrative plant biology · 2025Article
- PagHB7/PagABF4-PagEPFL9 Module Regulates Stomatal Density and Drought Tolerance in Poplar.Plant biotechnology journal · 2025Article
- SnRK2s: Kinases or Substrates?Plants (Basel, Switzerland) · 2025Review
- How UV-B-activated UVR8 triggers LOX1 to close stomata: Unveiling a mechanism of photoreceptor-regulated enzyme activity in the cytoplasm.The Plant cell · 2025Article
- Cryo-EM structures of Arabidopsis CNGC1 and CNGC5 reveal molecular mechanisms underlying gating and calcium selectivity.Nature plants · 2025Article
- Analysis ofInternational journal of molecular sciences · 2025Article
- CPK1 activates CNGCs through phosphorylation for CaNature communications · 2025Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Multiple cyclic nucleotide-gated channels (CNGCs) are abscisic acid (ABA)-activated Ca2+ channels in Arabidopsis (Arabidopsis thaliana) guard cells. In particular, CNGC5, CNGC6, CNGC9, and CNGC12 are essential for ABA-specific cytosolic Ca2+ signaling and stomatal movements. However, the mechanisms underlying ABA-mediated regulation of CNGCs and Ca2+ signaling are still unknown. In this study, we identified the Ca2+-independent protein kinase OPEN STOMATA 1 (OST1) as a CNGC activator in Arabidopsis. OST1-targeted phosphorylation sites were identified in CNGC5, CNGC6, CNGC9, and CNGC12. These CNGCs were strongly inhibited by Ser-to-Ala mutations and fully activated by Ser-to-Asp mutations at the OST1-targeted sites. The overexpression of individual inactive CNGCs (iCNGCs) under the UBIQUITIN10 promoter in wild-type Arabidopsis conferred a strong dominant-negative-like ABA-insensitive stomatal closure phenotype. In contrast, expressing active CNGCs (aCNGCs) under their respective native promoters in the cngc5-1 cngc6-2 cngc9-1 cngc12-1 quadruple mutant fully restored ABA-activated cytosolic Ca2+ oscillations and Ca2+ currents in guard cells, and rescued the ABA-insensitive stomatal movement mutant phenotypes. Thus, we uncovered that ABA elicits cytosolic Ca2+ signaling via an OST1-CNGC module, in which OST1 functions as a convergence point of the Ca2+-dependent and -independent pathways in Arabidopsis guard cells.
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