Evidence map›Paper›PMID 38441799›Full record

ArticleMolecular biotechnology2025

Heterologous Expression of Phycocyanobilin in Escherichia coli and Determination of Its Antioxidant Capacity In Vitro.

Ziying Ye, Jun Fang, Bin Yao, Gang Liu

Abstract read
PubMed Publisher
In one paragraph

Article in Molecular biotechnology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.9field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Ziying YeCollege of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, 410128, Hunan, China.
Jun FangCollege of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, 410128, Hunan, China. fangjun1973@hunau.edu.cn.ORCID http://orcid.org/0000-0001-9532-8444
Bin YaoCollege of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, 410128, Hunan, China.
Gang LiuCollege of Bioscience and Biotechnology, Hunan Agricultural University, Changsha, 410128, Hunan, China.
Hunan Agricultural University · CN

Funding

Double first-class construction project of Hunan Agricultural University SYL201802003Hunan Provincial Science and Technology Department 2016TP2005Hunan Provincial Science and Technology Department 2016WK2008Hunan Provincial Science and Technology Department 2017NK2322Hunan Provincial Science and Technology Department 2019TP2004Hunan Provincial Science and Technology Department 2020NK2004Hunan Provincial Science and Technology Department 2021JJ30008Postgraduate Scientific Research Innovation Project of Hunan Province CX20210654Science and Technology Innovation and Entrepreneurship Project for University Students of Hunan Province 2021RC1004
6 · The paper itself

Abstract

Phycocyanobilin (PCB) is a blue pigment with antioxidant, anti-inflammatory, and anticancer properties. It is used in the medical and cosmetic industries. In this study, a high-expression plasmid, pET-30a-PCB, was constructed for expression of PCB in Escherichia coli BL21(DE3). The PCB was analyzed using UV-visible absorption spectrum, MALDI-TOF-MS, and fluorescence spectra. The stability and half-life of PCB in different serum were determined. The yield of PCB was optimized through single-factor and orthogonal experiments. The optimal expression conditions were determined as a lactose concentration of 5 mmol/L, an induction time of 8 h, an induction temperature of 27 °C, and an induction duration of 22 h. PCB yield of 6.5 mg/L was achieved and subsequently purified using nickel-affinity chromatography. The purified PCB was quantified indirectly using Hist-tag ELISA detection, and the concentration was 11.66 μg/L. In the range of 0-33 μg/mL, the total antioxidant capacity and reducing the capacity of PCB were stronger than Vitamin E (Ve), with 1,1-diphenyl-2-picrylhydrazil (DPPH) scavenging reaching up to 87.07%, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) free radical (ABTS) scavenging up to 100%, hydroxyl radicals (·OH) scavenging up to 64.19%, hydrogen peroxide (H

Indexed as

AntioxidantsEscherichia coliPhycobilinsPhycocyaninRecombinant ProteinsAntioxidantsPhycobilinsPhycocyaninphycocyanobilinRecombinant ProteinsAntioxidant propertiesCondition optimizationEscherichia coliPhycocyanobilin

Identifiers

PMID38441799
OpenAlexW4392458060

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.