Investigating Protein-Protein Interactions of Autophagy-Involved TNIP1.

Michael L Samulevich, Liam E Carman, Brian J Aneskievich

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

4.0field-weighted citation impact, top 11% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

Conformational Analyses of the AHD1-UBAN Region of TNIP1 Highlight Key Amino Acids for Interaction with Ubiquitin.Biomolecules · 2025
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Michael L SamulevichGraduate Program in Pharmacology & Toxicology, University of Connecticut, Storrs, CT, USA.

Liam E CarmanGraduate Program in Pharmacology & Toxicology, University of Connecticut, Storrs, CT, USA.

Brian J AneskievichDepartment of Pharmaceutical Sciences, School of Pharmacy, University of Connecticut, Storrs, CT, USA. brian.aneskievich@uconn.edu.

University of Connecticut · US

Funding

DoD CDMRP grant W81XWH-20-1-0081

6 · The paper itself

Abstract

Myriad proteins are involved in the process of autophagy, which they participate in via their protein-protein interactions (PPI). Herein we outline a methodology for examining such interactions utilizing the case of intrinsically disordered protein (IDP) TNIP1 and its interaction with linear M1-linked polyubiquitin. This includes methods for recombinant production, purification, immuno-identification, and analysis of an IDP associated with autophagy, its ordered binding partner, and means of quantitatively analyzing their interaction.

Indexed as

AutophagyDNA-Binding ProteinsIntrinsically Disordered ProteinsProtein Interaction MappingHumansPolyubiquitinProtein BindingProtein Interaction MapsDNA-Binding ProteinsIntrinsically Disordered ProteinsPolyubiquitinBacterial expressionHeat lysisIntrinsically disordered proteinMicroscale thermophoresisNickel affinity chromatographyRecombinant protein

Identifiers

PMID38441723

DOI10.1016/0196-9781(90)90128-r

OpenAlexW4392476647

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.