ArticleJournal of animal science and biotechnology2024
Decreased eggshell strength caused by impairment of uterine calcium transport coincide with higher bone minerals and quality in aged laying hens.
Article in Journal of animal science and biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
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Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it, 44 citations in OpenAlex.
- Effects of microalgae supplementation on performance, egg quality, yolk fatty acid levels, and blood parameters in laying hens: a meta-analysis.Scientific reports · 2025Pooled it
- Effects of Oligomeric Ultrafine-Nano Hydrogen Water on Laying Performance, Egg Quality, Nutrient Composition, and Intestinal and Reproductive Responses in Late-Laying Hens.Animals : an open access journal from MDPI · 2026Article
- Phosphorus alleviates age-associated osteoporosis in laying hens by reshaping bone metabolism and modulating cecal microbial communities.Poultry science · 2026Article
- Plasma calcium, phosphorus, and calcium-to-phosphorus ratio as indicators of eggshell quality and reproductive performance in captive female Houbara bustards (<em>Chlamydotis undulata</em>).Veterinary world · 2026Article
- A Genome-Wide Association Study Exploring the Genetic Architecture of Eggshell Quality in Chinese Wenshang Barred Chickens.Animals : an open access journal from MDPI · 2026Article
- Effects of Broiler Breeder Age on Egg Quality, Incubation Performance, and Early Progeny Physiological and Muscle Fiber Development.Journal of animal physiology and animal nutrition · 2026Article
- Deciphering the uterine transcriptional mechanisms underlying eggshell strength through multi-stage analysis in laying hens.Poultry science · 2026Article
- Soybean isoflavones enhance production and albumen quality in aged laying hens by modulating ovarian function, immunity, and antioxidant capacity.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2026Article
- Dynamic synergistic interplay between ovarian antioxidant defense and angiogenesis sustains high egg production in laying hens.Journal of animal science and biotechnology · 2026Article
- Multi-tissue transcriptomic profiling reveals the internal physiological landscape of laying hens in cage and cage-free systems.Poultry science · 2026Article
- Effects of Dietary Supplementation with α-Mangostin on Oviduct Inflammation and Eggshell Quality in Aging Laying Hens.Animals : an open access journal from MDPI · 2026Article
- Optimizing eggshell quality in extended laying periods: Insights and strategies.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2026Review
- Laying hen responses to balanced protein reduction on performance, egg quality, nitrogen balance, and fat and mineral utilization.Journal of animal science · 2026Article
- Uterine fluid proteomic and metabolomic profiles associated with soft-shelled egg formation in laying hens.Frontiers in veterinary science · 2026Article
- Baicalin alleviates uterine inflammaging and improves eggshell quality in aged laying hens.Frontiers in immunology · 2026Article
- Disequilibrium feeding pattern more consistent with egg-laying physiology improves eggshell quality of laying hens during late laying period.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2025Article
- Regulatory effects of hawthorn leaf flavonoids and stevioside on the uterine function and eggshell quality in laying hens.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2025Article
- Redox status regulates eggshell color by modulating protoporphyrin IX biosynthesis via the SIRT1/PGC-1α/ALAS1 axis in brown-shelled hens.Journal of animal science and biotechnology · 2025Article
- Evaluating the efficacy of modified dried vinasse as a sustainable protein source for laying hens in the late phase of production.Tropical animal health and production · 2025Article
- Fermented Chinese Herbs Improved Egg Production, Egg Shell Quality, and Egg Yolk Cholesterol of Laying Hens by Regulating Estrogen, Lipid Metabolism, and Calcium Metabolism.Animals : an open access journal from MDPI · 2025Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundDeteriorations in eggshell and bone quality are major challenges in aged laying hens. This study compared the differences of eggshell quality, bone parameters and their correlations as well as uterine physiological characteristics and the bone remodeling processes of hens laying eggs of different eggshell breaking strength to explore the mechanism of eggshell and bone quality reduction and their interaction. A total of 240 74-week-old Hy-line Brown laying hens were selected and allocated to a high (HBS, 44.83 ± 1.31 N) or low (LBS, 24.43 ± 0.57 N) eggshell breaking strength group.
resultsA decreased thickness, weight and weight ratio of eggshells were observed in the LBS, accompanied with ultrastructural deterioration and total Ca reduction. Bone quality was negatively correlated with eggshell quality, marked with enhanced structures and increased components in the LBS. In the LBS, the mammillary knobs and effective layer grew slowly. At the initiation stage of eggshell calcification, a total of 130 differentially expressed genes (DEGs, 122 upregulated and 8 downregulated) were identified in the uterus of hens in the LBS relative to those in the HBS. These DEGs were relevant to apoptosis due to the cellular Ca overload. Higher values of p62 protein level, caspase-8 activity, Bax protein expression and lower values of Bcl protein expression and Bcl/Bax ratio were seen in the LBS. TUNEL assay and hematoxylin-eosin staining showed a significant increase in TUNEL-positive cells and tissue damages in the uterus of the LBS. Although few DEGs were identified at the growth stage, similar uterine tissue damages were also observed in the LBS. The expressions of runt-related transcription factor 2 and osteocalcin were upregulated in humeri of the LBS. Enlarged diameter and more structural damages of endocortical bones and decreased ash were observed in femurs of the HBS.
conclusionThe lower eggshell breaking strength may be attributed to a declined Ca transport due to uterine tissue damages, which could affect eggshell calcification and lead to a weak ultrastructure. Impaired uterine Ca transport may result in reduced femoral bone resorption and increased humeral bone formation to maintain a higher mineral and bone quality in the LBS.
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