ArticleVirology journal2024
Development and application of an indirect ELISA for detection of antibodies against emerging atypical porcine pestivirus.
Article in Virology journal, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers, 1 of them a synthesis that pooled it.
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Who cites it
3 citing papers in PubMed, 1 synthesis or guideline pooled it, 1 citations in OpenAlex.
- Prevalence of Getah Virus in Mammals in East and Southeast Asia: A Systematic Review and Meta-Analysis.Transboundary and emerging diseases · 2026Pooled it
- Development and validation of an E2 based indirect ELISA for serological detection of porcine Getah virus.Veterinary research communications · 2026Article
- Viremia and antibody dynamics following atypical porcine pestivirus infection: a cohort study of pigs with congenital tremor.Porcine health management · 2026Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
backgroundAtypical porcine pestivirus (APPV) is a newly discovered swine pestivirus, which can cause congenital tremor and high mortality in newborn piglets and subclinical infection in adult pigs, leading to significant impacts on the pig industry. Currently, there is no approved serological method to assess APPV infection status in pig farms.
methodsIn this study, the envelope glycoprotein E2 of APPV was highly expressed in suspension HEK293 cells, and further an indirect enzyme-linked immunosorbent assay based on the recombinant E2 protein (E2-iELISA) was developed and evaluated.
resultsThe reaction parameters of the E2-iELISA were optimized, and the cutoff value was determined to be 0.2 by analyzing S/P values of 165 negative sera against APPV that were confirmed by virus neutralization test (VNT). Specificity test showed that the method had no cross-reaction with other common swine viruses. The E2-iELISA was evaluated using a panel of swine sera, and showed high sensitivity (113/120, 94.2%) and specificity (65/70, 92.9%), and the agreement rate with VNT was 93.7% (178/190). Subsequently, the E2-iELISA was utilized to investigate the seroprevalence of APPV in pig herds of China. When detecting 1368 pig serum samples collected from nine provinces in China, the overall seroprevalence of APPV was 73.9% (1011/1368).
conclusionOur findings suggest that the E2-iELISA is specific and sensitive, and could be a valuable tool for serological surveillance of APPV infection in pigs.
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