ArticleNature communications2024
Light-field flow cytometry for high-resolution, volumetric and multiparametric 3D single-cell analysis.
Article in Nature communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 40 citations in OpenAlex.
- Nuclear mechanotransduction: tools for mechanical perturbation and chromatin characterization.Nucleus (Austin, Tex.) · 2026Review
- Rapid robust high-fidelity 3D neuronal extraction from multiview calcium imaging datasets.Nature methods · 2026Article
- Cell-based therapies of autoimmune diseases in the context of artificial intelligence development.Clinical and experimental medicine · 2026Review
- Single-Cell Protein Assays in Context: From 2D to 3D and In Situ Analysis.Annual review of analytical chemistry (Palo Alto, Calif.) · 2026Review
- Patch deconvolution for Fourier light-field microscopy.Biophysical journal · 2026Article
- Exploring the 3D architecture of brain tissue using digital holographic microscopy.Biomedical optics express · 2026Article
- LFC-Lab on a chip · 2026Article
- A high-throughput, flow cytometry approach to measure phase behavior and exchange in biomolecular condensates.Nature communications · 2026Article
- Volumetric localization microscopy with deep learning.Nature communications · 2025Article
- Three-dimensional forward-scattering imaging flow cytometry system for single-cell analysis.APL photonics · 2025Article
- Dual-Channel Event Microscopy for Ultrafast Biological Imaging.bioRxiv : the preprint server for biology · 2025Article
- Application of process analytical technology for real-time monitoring of synthetic co-culture bioprocesses.Analytical and bioanalytical chemistry · 2025Review
- Intelligent single-cell manipulation: LLMs- and object detection-enhanced active-matrix digital microfluidics.Microsystems & nanoengineering · 2025Article
- Spatial microfluidic holographic integrated platform for label-free and high-dimensional analysis of cancer heterogeneity.Nature communications · 2025Article
- High-speed fluorescence lifetime imaging microscopy: techniques, applications, and prospects.Biophotonics discovery · 2025Review
- Article
- Multiscale and recursive unmixing of spatiotemporal rhythms for live-cell and intravital cardiac microscopy.Nature cardiovascular research · 2025Article
- Femtosecond laser microfabrication of a fully-integrated optofluidic device for 3D imaging flow cytometry.Scientific reports · 2025Article
- Review
- Review
Corrections and comments
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Authors and funding
16 authors at 1 institution in 1 country.
Funding
Abstract
Imaging flow cytometry (IFC) combines flow cytometry and fluorescence microscopy to enable high-throughput, multiparametric single-cell analysis with rich spatial details. However, current IFC techniques remain limited in their ability to reveal subcellular information with a high 3D resolution, throughput, sensitivity, and instrumental simplicity. In this study, we introduce a light-field flow cytometer (LFC), an IFC system capable of high-content, single-shot, and multi-color acquisition of up to 5,750 cells per second with a near-diffraction-limited resolution of 400-600 nm in all three dimensions. The LFC system integrates optical, microfluidic, and computational strategies to facilitate the volumetric visualization of various 3D subcellular characteristics through convenient access to commonly used epi-fluorescence platforms. We demonstrate the effectiveness of LFC in assaying, analyzing, and enumerating intricate subcellular morphology, function, and heterogeneity using various phantoms and biological specimens. The advancement offered by the LFC system presents a promising methodological pathway for broad cell biological and translational discoveries, with the potential for widespread adoption in biomedical research.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.