Evidence map›Paper›PMID 38435708›Full record

ArticleSynthetic and systems biotechnology2024

Construction of a broad-host-range Anderson promoter series and particulate methane monooxygenase promoter variants expand the methanotroph genetic toolbox.

Etash H Bhat, Jessica M Henard, Spencer A Lee, Dustin McHalffey, Mahith S Ravulapati, Elle V Rogers, Logan Yu, David Skiles, Calvin A Henard

Open access · goldAbstract read
In one paragraph

Article in Synthetic and systems biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
3.0field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 13 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Etash H BhatDepartment of Biological Sciences and BioDiscovery Institute, University of North Texas, Denton, TX, USA.
Jessica M HenardDepartment of Biological Sciences and BioDiscovery Institute, University of North Texas, Denton, TX, USA.
Spencer A LeeDepartment of Biological Sciences and BioDiscovery Institute, University of North Texas, Denton, TX, USA.
Dustin McHalffeyDepartment of Biological Sciences and BioDiscovery Institute, University of North Texas, Denton, TX, USA.
Mahith S RavulapatiDepartment of Biological Sciences and BioDiscovery Institute, University of North Texas, Denton, TX, USA.
Elle V RogersDepartment of Biological Sciences and BioDiscovery Institute, University of North Texas, Denton, TX, USA.
Logan YuDepartment of Biological Sciences and BioDiscovery Institute, University of North Texas, Denton, TX, USA.
David SkilesDepartment of Biological Sciences and BioDiscovery Institute, University of North Texas, Denton, TX, USA.
Calvin A HenardDepartment of Biological Sciences and BioDiscovery Institute, University of North Texas, Denton, TX, USA.
University of North Texas · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Methanotrophic bacteria are currently used industrially for the bioconversion of methane-rich natural gas and anaerobic digestion-derived biogas to valuable products. These bacteria may also serve to mitigate the negative effects of climate change by capturing atmospheric greenhouse gases. Several genetic tools have previously been developed for genetic and metabolic engineering of methanotrophs. However, the available tools for use in methanotrophs are significantly underdeveloped compared to many other industrially relevant bacteria, which hinders genetic and metabolic engineering of these biocatalysts. As such, expansion of the methanotroph genetic toolbox is needed to further our understanding of methanotrophy and develop biotechnologies that leverage these unique microbes for mitigation and conversion of methane to valuable products. Here, we determined the copy number of three broad-host-range plasmids in

Indexed as

Metabolic engineeringMethane monooxygenaseMethanotrophPromoterSynthetic biology

Identifiers

PMID38435708
PMCPMC10909576
OpenAlexW4391933753

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.