ArticleACS omega2024
Investigating and Optimizing Insulin Partitioning with Conjugated Au Nanoparticles in Aqueous Two-Phase Systems Using Response Surface Methodology.
Article in ACS omega, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
This study investigated the impact of bioconjugation on the partitioning of insulin, a clinically valuable protein, in an aqueous two-phase system. Gold nanoparticles of different sizes were synthesized and conjugated with insulin. Analysis of the conjugated insulin showed that the insulin remains fully active. Conjugated gold nanoparticles (AuNPs/insulin) were used in polyethylene glycol (PEG)-dextran aqueous two-phase systems to investigate the effect of pH, PEG and dextran molecular weights, PEG and dextran concentrations, AuNPs/insulin dosage, and nanoparticle size on the partition coefficient. These systems were chosen for their biocompatibility and low toxicity. Response surface methodology with D-optimal design was used to model and optimize these systems and their affected parameters. At the optimum condition of a pH = 8 system containing 21% PEG 4000, 5% dextran 100,000, and 100 IU AuNPs/insulin, the partition coefficient of AuNPs/insulin was found to be 192.96, which is in agreement with the empirical partition coefficient of 189.2. This is significantly higher than the partition coefficient of free insulin in a similar system. This approach could be used to overcome limitations in the feasibility of aqueous two-phase systems for industrial-scale purification of biomolecules and biopharmaceuticals.
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