Evidence map›Paper›PMID 38433217›Full record

ArticleStem cell research & therapy2024

Improving hematopoietic differentiation from human induced pluripotent stem cells by the modulation of Hippo signaling with a diarylheptanoid derivative.

Umnuaychoke Thongsa-Ad, Anongnat Wongpan, Wasinee Wongkummool, Phaewa Chaiwijit, Kwanchanok Uppakara, Gorawin Chaiyakitpattana, Passanan Singpant, Pirut Tong-Ngam, Amnat Chukhan, Wachirachai Pabuprappap and 7 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell research & therapy, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.3field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors at 4 institutions in 1 country.

Umnuaychoke Thongsa-AdDepartment of Physiology, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand.
Anongnat WongpanDepartment of Physiology, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand.
Wasinee WongkummoolStem Cell Research Group, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, 73170, Thailand.
Phaewa ChaiwijitDepartment of Physiology, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand.
Kwanchanok UppakaraChakri Naruebodindra Medical Institute, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Samut Prakan, 10540, Thailand.
Gorawin ChaiyakitpattanaDepartment of Physiology, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand.
Passanan SingpantStem Cell Research Group, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, 73170, Thailand.
Pirut Tong-NgamStem Cell Research Group, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, 73170, Thailand.
Amnat ChukhanPrima Scientific, 147/170-171 Baromrajchonnee, Arunamarin, Bangkok, 10700, Thailand.
Wachirachai PabuprappapDepartment of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Ramkhamhaeng University, Bangkok, 10240, Thailand.
Sirapope WongniamCenter for Scientific Instrumentation and Platform Services Unit, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand.
Apichart SuksamrarnDepartment of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Ramkhamhaeng University, Bangkok, 10240, Thailand.
Suradej HongengDepartment of Pediatrics, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, 10400, Thailand.
Usanarat AnurathapanDepartment of Pediatrics, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, 10400, Thailand.
Kasem KulkeawSiriraj Integrative Center for Neglected Parasitic Diseases, Department of Parasitology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, 10700, Thailand.
Alisa TubsuwanStem Cell Research Group, Institute of Molecular Biosciences, Mahidol University, Nakhon Pathom, 73170, Thailand.
Kanit BhukhaiDepartment of Physiology, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand. kanit.bhu@mahidol.ac.th.
Mahidol University · THRamathibodi Hospital · THRamkhamhaeng University · THChiang Mai University · TH

Funding

Faculty of Science, Mahidol University the Central Instrument Facility (CIF)Mahidol University MU's Strategic Research Fund: fiscal year 2023Ministry of Science and Technology of Thailand the Science Achievement Scholarship of ThailandNational Research Council of Thailand N41A660154
6 · The paper itself

Abstract

backgroundThe diarylheptanoid ASPP 049 has improved the quality of adult hematopoietic stem cell (HSC) expansion ex vivo through long-term reconstitution in animal models. However, its effect on hematopoietic regeneration from human induced pluripotent stem cells (hiPSCs) is unknown.

methodWe utilized a defined cocktail of cytokines without serum or feeder followed by the supplementation of ASPP 049 to produce hematopoietic stem/progenitor cells (HSPCs). Flow cytometry and trypan blue exclusion analysis were used to identify nonadherent and adherent cells. Nonadherent cells were harvested to investigate the effect of ASPP 049 on multipotency using LTC-IC and CFU assays. Subsequently, the mechanism of action was explored through transcriptomic profiles, which were validated by qRT-PCR, immunoblotting, and immunofluorescence analysis.

resultThe supplementation of ASPP 049 increased the number of phenotypically defined primitive HSPCs (CD34

conclusionThese findings suggest that ASPP 049 can improve HSC-generating protocols with proliferative potentials, self-renewal ability, unbiased differentiation, and a definable mechanism of action for the clinical perspective of hematopoietic regenerative medicine.

Indexed as

Hippo Signaling PathwayInduced Pluripotent Stem CellsAdultAnimalsAntigens, CD34Cell DifferentiationDiarylheptanoidsHumansAntigens, CD34ASPP 049DiarylheptanoidsDiarylheptanoidHippo signaling pathwayHuman induced pluripotent stem cellsPrimitive hematopoietic stem and progenitor cells

Identifiers

PMID38433217
PMCPMC10910864
OpenAlexW4392342769

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.