Evidence map›Paper›PMID 38430669›Full record

ArticleJournal of clinical virology : the official publication of the Pan American Society for Clinical Virology2024

Performance of the Xpert™ Mpox PCR assay with oropharyngeal, anorectal, and cutaneous lesion swab specimens.

Gregory L Damhorst, Kaleb McLendon, Evelyn Morales, Zianya M Solis, Eric Fitts, Heather B Bowers, Courtney Sabino, Julie Sullivan, Morgan Greenleaf, John D Roback and 7 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Article
  3. Observational
  4. Article
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Gregory L DamhorstDivision of Infectious Diseases, Department of Medicine, Emory University, Atlanta, GA, USA; The Atlanta Center for Microsystems-Engineered Point-of-Care Technologies, Atlanta, Georgia, USA; Ponce de Leon Center, Grady Health System, Atlanta, Georgia, USA.
Kaleb McLendonDepartment of Pathology and Laboratory Medicine, Emory University, Atlanta, GA, USA.
Evelyn MoralesDepartment of Pathology and Laboratory Medicine, Emory University, Atlanta, GA, USA.
Zianya M SolisDepartment of Pathology and Laboratory Medicine, Emory University, Atlanta, GA, USA.
Eric FittsDepartment of Pathology and Laboratory Medicine, Emory University, Atlanta, GA, USA.
Heather B BowersThe Atlanta Center for Microsystems-Engineered Point-of-Care Technologies, Atlanta, Georgia, USA; Department of Pediatrics, Emory University, Atlanta, GA, USA; Laboratory of Biochemical Pharmacology, Emory University, Atlanta, Georgia, USA.
Courtney SabinoThe Atlanta Center for Microsystems-Engineered Point-of-Care Technologies, Atlanta, Georgia, USA; Department of Pediatrics, Emory University, Atlanta, GA, USA; Laboratory of Biochemical Pharmacology, Emory University, Atlanta, Georgia, USA.
Julie SullivanThe Atlanta Center for Microsystems-Engineered Point-of-Care Technologies, Atlanta, Georgia, USA.
Morgan GreenleafThe Atlanta Center for Microsystems-Engineered Point-of-Care Technologies, Atlanta, Georgia, USA.
John D RobackDepartment of Pathology and Laboratory Medicine, Emory University, Atlanta, GA, USA.
Jonathan A ColasantiDivision of Infectious Diseases, Department of Medicine, Emory University, Atlanta, GA, USA; Ponce de Leon Center, Grady Health System, Atlanta, Georgia, USA.
Anandi N ShethDivision of Infectious Diseases, Department of Medicine, Emory University, Atlanta, GA, USA; Ponce de Leon Center, Grady Health System, Atlanta, Georgia, USA.
Boghuma K TitanjiDivision of Infectious Diseases, Department of Medicine, Emory University, Atlanta, GA, USA; Ponce de Leon Center, Grady Health System, Atlanta, Georgia, USA.
Greg S MartinThe Atlanta Center for Microsystems-Engineered Point-of-Care Technologies, Atlanta, Georgia, USA; Division of Pulmonary, Allergy, Critical Care, and Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, USA.
Leda BassitThe Atlanta Center for Microsystems-Engineered Point-of-Care Technologies, Atlanta, Georgia, USA; Department of Pediatrics, Emory University, Atlanta, GA, USA; Laboratory of Biochemical Pharmacology, Emory University, Atlanta, Georgia, USA.
Wilbur A LamThe Atlanta Center for Microsystems-Engineered Point-of-Care Technologies, Atlanta, Georgia, USA; Department of Pediatrics, Emory University, Atlanta, GA, USA; Aflac Cancer & Blood Disorders Center at Children's Healthcare of Atlanta, Atlanta, Georgia, USA; Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, Georgia, USA.
Anuradha RaoThe Atlanta Center for Microsystems-Engineered Point-of-Care Technologies, Atlanta, Georgia, USA; Department of Pediatrics, Emory University, Atlanta, GA, USA. Electronic address: anuradha.rao@emory.edu.

Funding

Technology Training and Dissemination CoreU54EB027690 · NIBIB · EMORY UNIVERSITY · PI Wilbur A Lam · 2018 to 2026
$96.1M
Virology and Molecular Biomarkers CoreP30AI050409 · NIAID · EMORY UNIVERSITY · PI Ann M Chahroudi, Colleen F Kelley · 2002 to 2026
$74.0M
RADx Innovation Funnel for HIV diagnostics – Validation Center75N92022D00015 · NIBIB · EMORY UNIVERSITY · PI PARSLOW, TRISTRAM G. · 2022 to 2025
$19.9M
NHLBI NIH HHS 75N92022D00015NIAID NIH HHS P30 AI050409NIBIB NIH HHS U54 EB027690
6 · The paper itself

Abstract

Anorectal and oropharyngeal exposures are implicated in sexual transmission of mpox, but authorized assays in the United States are only validated with cutaneous lesion swabs. Diagnostic assays for anorectal and oropharyngeal swabs are needed to address potential future outbreaks. The Cepheid Xpert® Mpox is the first point-of-care assay to receive FDA emergency use authorization in the United States and would be a valuable tool for evaluating these sample types. Our exploratory study demonstrates 100 % positive agreement with our in-house PCR assay for natural positive anorectal and oropharyngeal specimens and 92 % sensitivity with low-positive spiked specimens. The Xpert® assay detected viral DNA in specimens not detected by our reference PCR assay from four participants with mpox DNA at other sites, suggesting it may be more sensitive at low viral loads. In conclusion, the validation of the Xpert® for oropharyngeal and anorectal sample types can be rapidly achieved if clinical need returns and prospective samples become available.

Indexed as

Mpox, MonkeypoxHumansPolymerase Chain ReactionProspective StudiesSensitivity and SpecificitySpecimen HandlingUnited StatesAnorectal swabDiagnosticMpoxOropharyngeal swabPoint-of-careSexually transmitted infections

Identifiers

PMID38430669
PMCPMC11160946

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.