Evidence map›Paper›PMID 38429619›Full record

ArticleFish physiology and biochemistry2024

Evolutionary analysis of SLC10 family members and insights into function and expression regulation of lamprey NTCP.

Yingying Zhu, Qipeng Zhang, Jilong Pan, Tiesong Li, Hao Wang, Jindi Liu, Lei Qian, Ting Zhu, Yue Pang, Qingwei Li and 1 more

Abstract read
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In one paragraph

Article in Fish physiology and biochemistry, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.1field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Hepatitis B and D virus entry.Nature reviews. Microbiology · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 1 country.

Yingying Zhu *College of Life Sciences, Liaoning Normal University, Dalian116081, China.
Qipeng Zhang *College of Life Sciences, Liaoning Normal University, Dalian116081, China.
Jilong PanCollege of Life Sciences, Liaoning Normal University, Dalian116081, China.
Tiesong LiCollege of Life Sciences, Liaoning Normal University, Dalian116081, China.
Hao WangCollege of Life Sciences, Liaoning Normal University, Dalian116081, China.
Jindi LiuCollege of Life Sciences, Liaoning Normal University, Dalian116081, China.
Lei QianThe Advanced Institute for Medical Sciences, Dalian Medical University, Dalian, 116044, China.
Ting ZhuCollege of Life Sciences, Liaoning Normal University, Dalian116081, China.
Yue PangCollege of Life Sciences, Liaoning Normal University, Dalian116081, China.
Qingwei LiCollege of Life Sciences, Liaoning Normal University, Dalian116081, China.
Yan ChiCollege of Life Sciences, Liaoning Normal University, Dalian116081, China. chiyan@lnnu.edu.cn.
Liaoning Normal University · CNDalian Medical University · CN

Funding

Doctoral scientific research foundation of Liaoning Normal University, China BS2020L002Education Department of Liaoning Province, China LF2020005Liaoning province natural science and technology plan guidance project, China 2019-ZD-0301
6 · The paper itself

Abstract

The Na ( +)-taurocholate cotransporting polypeptide (NTCP) is a member of the solute carrier family 10 (SLC10), which consists of 7 members (SLC10a1-SLC10a7). NTCP is a transporter localized to the basolateral membrane of hepatocytes and is primarily responsible for the absorption of bile acids. Although mammalian NTCP has been extensively studied, little is known about the lamprey NTCP (L-NTCP). Here we show that L-NTCP follows the biological evolutionary history of vertebrates, with conserved domain, motif, and similar tertiary structure to higher vertebrates. L-NTCP is localized to the cell surface of lamprey primary hepatocytes by immunofluorescence analysis. HepG2 cells overexpressing L-NTCP also showed the distribution of L-NTCP on the cell surface. The expression profile of L-NTCP showed that the expression of NTCP is highest in lamprey liver tissue. L-NTCP also has the ability to transport bile acids, consistent with its higher vertebrate orthologs. Finally, using a farnesoid X receptor (FXR) antagonist, RT-qPCR and flow cytometry results showed that L-NTCP is negatively regulated by the nuclear receptor FXR. This study is important for understanding the adaptive mechanisms of bile acid metabolism after lamprey biliary atresia based on understanding the origin, evolution, expression profile, biological function, and expression regulation of L-NTCP.

Indexed as

LampreysOrganic Anion Transporters, Sodium-DependentSymportersAmino Acid SequenceAnimalsBile Acids and SaltsEvolution, MolecularFish ProteinsGene Expression RegulationHepatocytesHep G2 CellsHumansPhylogenyBile Acids and SaltsFish ProteinsOrganic Anion Transporters, Sodium-Dependentsodium-bile acid cotransporterSymportersBiliary atresiaFarnesoid X receptorLampreyNa ( +)-taurocholate cotransporting polypeptide

Identifiers

PMID38429619
OpenAlexW4392346289

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.