Isolation and Detection of Pathological Tau Species in a Tauopathy Mouse Model.

Abhay Kumar Singh, Karthikeyan Selvarasu, Siva Sundara Kumar Durairajan

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Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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3 authors at 1 institution in 1 country.

Abhay Kumar SinghMolecular Mycology and Neurodegenerative Disease Research Laboratory, Department of Microbiology, Central University of Tamil Nadu, Thiruvarur, India.

Karthikeyan SelvarasuMolecular Mycology and Neurodegenerative Disease Research Laboratory, Department of Microbiology, Central University of Tamil Nadu, Thiruvarur, India.

Siva Sundara Kumar DurairajanMolecular Mycology and Neurodegenerative Disease Research Laboratory, Department of Microbiology, Central University of Tamil Nadu, Thiruvarur, India. d.sivasundarakumar@cutn.ac.in.

Central University of Tamil Nadu · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Tau protein in Alzheimer's disease (AD) and tauopathies becomes insoluble due to hyperphosphorylation, conformational alterations, and aggregation. To analyze insoluble tau and pathological tau species, this study employs a methodology that utilizes wild-type and transgenic tau mice (P310S Tau) tissue extraction using 1% Sarkosyl or N-Lauroylsarcosine sodium salt and the radio immunoprecipitation assay (RIPA) buffer. However, the commonly used methods to study the insoluble tau fraction using detergents like Sarkosyl and RIPA require a large amount of homogenate, which can pose challenges when dealing with small tissue samples. Additionally, the study employs immunohistochemistry to visualize and quantify the pathological tau species in the brain tissue of transgenic mice, aiming to identify and analyze pathological tau species such as hyperphosphorylated tau to further our understanding of tauopathies such as Alzheimer's disease.

Indexed as

Alzheimer DiseaseTauopathiesAnimalsBrainDisease Models, AnimalMiceMice, Transgenictau Proteinstau ProteinsImmunohistochemistryInsoluble phosphotauTauopathiesTransgenic tau mouse modelUltracentrifugationWestern blotting

Identifiers

PMID38427247

DOI10.1016/j.neurobiolaging.2012.02.016

OpenAlexW4392398036

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.