Evidence map›Paper›PMID 38427186›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

CSF N-Glycomics Using High-Throughput UPLC-ESI Techniques in Alzheimer's Disease.

Angela Messina, Rita Barone, Luisa Sturiale, Mario Zappia, Angelo Palmigiano, Domenico Garozzo

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 94% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Angela MessinaCNR, Istituto per i Polimeri, Compositi e i Biomateriali Catania, Catania, Italy.
Rita BaroneCNR, Istituto per i Polimeri, Compositi e i Biomateriali Catania, Catania, Italy.
Luisa SturialeCNR, Istituto per i Polimeri, Compositi e i Biomateriali Catania, Catania, Italy.
Mario ZappiaSection of Neurosciences-Department GF Ingrassia, University of Catania, Catania, Italy.
Angelo PalmigianoCNR, Istituto per i Polimeri, Compositi e i Biomateriali Catania, Catania, Italy. angelo.palmigiano@cnr.it.
Domenico GarozzoCNR, Istituto per i Polimeri, Compositi e i Biomateriali Catania, Catania, Italy. domenico.garozzo@cnr.it.
Institute of Polymers, Composites and Biomaterials · ITUniversity of Catania · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In this chapter, we will present a high-throughput method applied in our laboratory for the structural elucidation of the cerebrospinal fluid (CSF) N-glycome. This methodology is based on a commercial equipment developed by WATERS™ to speed up N-deglycosylation and N-glycan labeling of glycoproteins of pharmaceutical and biological interest such as monoclonal antibodies. This analytical kit is sold under the trade name of RapiFluor-MS (RFMS). We have slightly modified the methodology, increasing the glycosylation time and using a high-resolution mass analyzer for the analysis of CSF N-glycans, thus obtaining a high-throughput method (up to 96 samples simultaneously), mass accuracy better than 5 ppm, and the ability to separate and identify isomers.

Indexed as

Alzheimer DiseaseGlycomicsChromatography, High Pressure LiquidGlycoproteinsGlycosylationHumansPolysaccharidesGlycoproteinsPolysaccharidesADGlyco-biomarkersHigh-throughput N-glycomicsN-glycans

Identifiers

PMID38427186
OpenAlexW4392398438

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.