Evidence map›Paper›PMID 38427097›Full record

ArticleMolecular biology reports2024

Molecular characterization of Chikungunya virus recovered from patients in the Maranhão state, Brazil.

Antônio Dantas Silva Junior, Bruna Oliveira de Melo, Antonia Khaynnam Silva Costa, Daniela de Jesus Ferreira Costa, Érima Jôyssielly Mendonça Castro, Rosimary de Jesus Gomes Turri, Andrea de Sousa Monteiro, Adrielle Zagmignan, Maria Rosa Quaresma Bomfim, Luís Cláudio Nascimento de Silva

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Article in Molecular biology reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 2 countries.

Antônio Dantas Silva JuniorLaboratório de Patogenicidade Microbiana, Universidade CEUMA, São Luís, MA, 65075-120, Brazil.
Bruna Oliveira de MeloLaboratório de Microbiologia Aplicada, Universidade CEUMA, São Luís, MA, 65075-120, Brazil.
Antonia Khaynnam Silva CostaLaboratório de Microbiologia Aplicada, Universidade CEUMA, São Luís, MA, 65075-120, Brazil.
Daniela de Jesus Ferreira CostaLaboratório de Microbiologia Aplicada, Universidade CEUMA, São Luís, MA, 65075-120, Brazil.
Érima Jôyssielly Mendonça CastroLaboratório de Patogenicidade Microbiana, Universidade CEUMA, São Luís, MA, 65075-120, Brazil.
Rosimary de Jesus Gomes TurriDepartamento de Farmácia, Universidade Federal do Maranhão, São Luís, MA, 65085-582, Brazil.
Andrea de Sousa MonteiroLaboratório de Microbiologia Aplicada, Universidade CEUMA, São Luís, MA, 65075-120, Brazil.
Adrielle ZagmignanLaboratório de Patogenicidade Microbiana, Universidade CEUMA, São Luís, MA, 65075-120, Brazil.
Maria Rosa Quaresma BomfimLaboratório de Microbiologia Aplicada, Universidade CEUMA, São Luís, MA, 65075-120, Brazil.
Luís Cláudio Nascimento de SilvaLaboratório de Patogenicidade Microbiana, Universidade CEUMA, São Luís, MA, 65075-120, Brazil. luisclaudionsilva@yahoo.com.br.
Universidade Ceuma · BRUniversidade Federal do Maranhão · BRUniversity of Lusaka · ZM

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundChikungunya virus (CHIKV) is an arbovirus from the Togaviridae family which has four genotypes: West African (WA), East/Central/South African (ECSA) and Asian/Caribbean lineage (AL) and Indian Ocean Lineage (IOL). The ECSA genotype was first registered in Brazil in Feira de Santana and spread to all Brazilian regions. This study reports the characterization of CHIKV isolates recovered from sera samples of fifty patients from seventeen cities in Maranhão, a state from Brazilian northeast region and part of the Legal Amazon area. METHODS AND

resultsPrimers were developed to amplify the partial regions coding structural proteins (E1, E3, E2, 6 K, and Capsid C). The consensus sequences have 2871 bp, covering approximately 24% of the genome. The isolates were highly similar (> 99%) to the ECSA isolate from Feira de Santana (BHI3734/H804698), presenting 30 non-synonymous mutations in E1 (5.95%), 18 in E2 (4.46%), and 1 in E3 (3.03%), taking the BHI3734/H804698 isolate as standard. Although the mutations described have not previously been related to increased infectivity or transmissibility of CHIKV, in silico analysis showed changes in physicochemical characteristics, antigenicity, and B cell epitopes of E1 and E2.

conclusionsThese findings demonstrate the importance of molecular approaches for monitoring the viral adaptations undergone by CHIKV and its geographic distribution.

Indexed as

Chikungunya FeverChikungunya virusBrazilDisease OutbreaksGenotypeHumansPhylogenyArbovirusesIn silico analysisMutationsPhylogenetic analysisStructural protein

Identifiers

PMID38427097
OpenAlexW4392365184

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.