ArticleISME communications2024
High amino acid osmotrophic incorporation by marine eukaryotic phytoplankton revealed by click chemistry.
Article in ISME communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 7 citations in OpenAlex.
- Advancements and applications of click chemistry in protein labeling and bioconjugation.RSC advances · 2026Review
- Mixotrophy emerges as an optimal strategy in mature waters of the Amazon River plume.Communications biology · 2026Article
- Photosynthetic and nutritional plasticity in mixotrophic dinoflagellates: responses to environmental change.Frontiers in plant science · 2026Review
- Single-cell heterotrophic activity in deep-ocean prokaryotic communities quantified by BONCAT and microautoradiography.ISME communications · 2026Article
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Authors and funding
13 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The osmotrophic uptake of dissolved organic compounds in the ocean is considered to be dominated by heterotrophic prokaryotes, whereas the role of planktonic eukaryotes is still unclear. We explored the capacity of natural eukaryotic plankton communities to incorporate the synthetic amino acid L-homopropargylglycine (HPG, analogue of methionine) using biorthogonal noncanonical amino acid tagging (BONCAT), and we compared it with prokaryotic HPG use throughout a 9-day survey in the NW Mediterranean. BONCAT allows to fluorescently identify translationally active cells, but it has never been applied to natural eukaryotic communities. We found a large diversity of photosynthetic and heterotrophic eukaryotes incorporating HPG into proteins, with dinoflagellates and diatoms showing the highest percentages of BONCAT-labelled cells (49 ± 25% and 52 ± 15%, respectively). Among them, pennate diatoms exhibited higher HPG incorporation in the afternoon than in the morning, whereas small (≤5 μm) photosynthetic eukaryotes and heterotrophic nanoeukaryotes showed the opposite pattern. Centric diatoms (e.g.
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Registered trials
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