ArticlePlant biotechnology journal2024
A method of genetic transformation and gene editing of succulents without tissue culture.
Article in Plant biotechnology journal, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Integrated transcriptomic and metabolomic analysis unveils biosynthetic mechanisms of key bioactive isoflavonoid accumulation inHorticulture research · 2026Article
- Magnetic nanoparticle-mediated genetic transformation and gene editing system in loquat (Eriobotrya japonica).Journal of integrative plant biology · 2026Article
- Bibliometric-Based Analysis of Global Trends and Collaborative Networks in Plant Genetic Engineering (1994-2024).Plant biotechnology journal · 2026Review
- An efficient tissue-culture-free soybean genetic transformation technology using the extremely simple cut-dip-budding strategy.Innovation (Cambridge (Mass.)) · 2026Review
- Transposon-derived genome editors in plants: from compact nucleases to large-fragment integration and regeneration strategies.Engineering in life sciences · 2026Review
- Next-generation CRISPR systems and advanced delivery paradigms for precision crop improvement: a comparative case study in sugar beet (Frontiers in plant science · 2026Review
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- Soybean Cyst Nematode-Resistant Protein AATPlant direct · 2025Article
- Forage Crop Research in the Modern Age.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- An efficient hairy root system for validation of CRISPR/Cas system activities in cotton.Functional & integrative genomics · 2025Article
- Highly efficient Agrobacterium rhizogenes-mediated gene editing system in Salvia miltiorrhiza inbred line bh2-7.Plant biotechnology journal · 2025Article
- Manipulating brassinosteroid signaling pathway to genetically improve horticultural plants.aBIOTECH · 2025Review
- Non-tissue culture genetic modifications for plant improvement.Plant molecular biology · 2025Review
- Current Advancement and Future Prospects in Simplified Transformation-Based Plant Genome Editing.Plants (Basel, Switzerland) · 2025Review
- Engineering Agrobacterium for improved plant transformation.The Plant journal : for cell and molecular biology · 2025Review
- Novel methods for genetic transformation of watermelon (Molecular breeding : new strategies in plant improvement · 2025Article
- Stable transformation mediated byFrontiers in plant science · 2025Article
- Tissue culture-independent approaches to revolutionizing plant transformation and gene editing.Horticulture research · 2025Article
- A simple and efficient system for evaluating plant genome editing efficiency and its application in optimizing the ISAam1 TnpB nuclease.Frontiers in plant science · 2025Article
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Authors and funding
12 authors.
Funding
Abstract
Succulents, valued for their drought tolerance and ornamental appeal, are important in the floriculture market. However, only a handful of succulent species can be genetically transformed, making it difficult to improve these plants through genetic engineering. In this study, we adapted the recently developed cut-dip-budding (CDB) gene delivery system to transform three previously recalcitrant succulent varieties - the dicotyledonous Kalanchoe blossfeldiana and Crassula arborescens and the monocotyledonous Sansevieria trifasciata. Capitalizing on the robust ability of cut leaves to regenerate shoots, these plants were successfully transformed by directly infecting cut leaf segments with the Agrobacterium rhizogenes strain K599. The transformation efficiencies were approximately 74%, 5% and 3.9%-7.8%, respectively, for K. blossfeldiana and C. arborescens and S. trifasciata. Using this modified CDB method to deliver the CRISPR/Cas9 construct, gene editing efficiency in K. blossfeldiana at the PDS locus was approximately 70%. Our findings suggest that succulents with shoot regeneration ability from cut leaves can be genetically transformed using the CDB method, thus opening up an avenue for genetic engineering of these plants.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.