ArticleBiotechnology for biofuels and bioproducts2024
Enhanced bacterial cellulose production in Komagataeibacter sucrofermentans: impact of different PQQ-dependent dehydrogenase knockouts and ethanol supplementation.
Article in Biotechnology for biofuels and bioproducts, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 29 citations in OpenAlex.
- Bioengineering microbial cellulose synthesis pathways as biofactory for industrial-scale cellulose production: a review on current advances.World journal of microbiology & biotechnology · 2026Review
- Article
- Biocircular economy-driven bacterial cellulose with large pore size: statistical optimization using Glutamicibacter soli bread waste hydrolysate.Bioresources and bioprocessing · 2026Article
- Comparative Genomics of High-Yielding Komagataeibacter diospyri Mutants Induced by Ultraviolet Light.Molecular biotechnology · 2026Article
- Effects of Fermentation Time and Temperature on the Physicochemical Quality of Kombucha.Foods (Basel, Switzerland) · 2026Article
- Microbial Valorization of Agricultural and Agro-Industrial Waste into Bacterial Cellulose: Innovations for Circular Bioeconomy Integration.Microorganisms · 2025Review
- Transcriptional reprogramming of Novacetimonas hansenii SI1 during growth on glycerol.Applied microbiology and biotechnology · 2025Article
- Construction of a synthetic biology toolkit for the genetic manipulation in the cellulose-producing strainSynthetic and systems biotechnology · 2025Article
- Genetic Impacts on the Structure and Mechanics of Cellulose Made by Bacteria.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Enhanced Bacterial Cellulose Production Using Hempseed Meal: Optimal Conditions and Properties.Biotech (Basel (Switzerland)) · 2025Article
- Bacterial Cellulose for Scalable and Sustainable Bio-Gels in the Circular Economy.Gels (Basel, Switzerland) · 2025Review
- Modulating Microbial Materials - Engineering Bacterial Cellulose with Synthetic Biology.ACS synthetic biology · 2024Review
- How carbon sources drive cellulose synthesis in two Komagataeibacter xylinus strains.Scientific reports · 2024Article
- Sustainability and Influencing Factors in Bacterial Cellulose Production: A Review of the Impact of Microorganisms, Culture Media and Cultivation Methods.Food technology and biotechnologyReview
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5 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundBacterial cellulose (BC) is a biocompatible material with unique mechanical properties, thus holding a significant industrial potential. Despite many acetic acid bacteria (AAB) being BC overproducers, cost-effective production remains a challenge. The role of pyrroloquinoline quinone (PQQ)-dependent membrane dehydrogenases (mDH) is crucial in the metabolism of AAB since it links substrate incomplete oxidation in the periplasm to energy generation. Specifically, glucose oxidation to gluconic acid substantially lowers environmental pH and hinders BC production. Conversely, ethanol supplementation is known to enhance BC yields in Komagataeibacter spp. by promoting efficient glucose utilization.
resultsK. sucrofermentans ATCC 700178 was engineered, knocking out the four PQQ-mDHs, to assess their impact on BC production. The strain KS003, lacking PQQ-dependent glucose dehydrogenase (PQQ-GDH), did not produce gluconic acid and exhibited a 5.77-fold increase in BC production with glucose as the sole carbon source, and a 2.26-fold increase under optimal ethanol supplementation conditions. In contrast, the strain KS004, deficient in the PQQ-dependent alcohol dehydrogenase (PQQ-ADH), showed no significant change in BC yield in the single carbon source experiment but showed a restrained benefit from ethanol supplementation.
conclusionsThe results underscore the critical influence of PQQ-GDH and PQQ-ADH and clarify the effect of ethanol supplementation on BC production in K. sucrofermentans ATCC 700178. This study provides a foundation for further metabolic pathway optimization, emphasizing the importance of diauxic ethanol metabolism for high BC production.
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