Evidence map›Paper›PMID 38420508›Full record

ArticleFood chemistry: X2024

A reliable enzyme-linked immunosorbent assay for the determining of sesame proteins in raw food ingredients and in processed foods.

Masayoshi Tomiki, Masatoshi Sakai, Daichi Tanaka, Mai Hosoya, Kana Uchida, Haruki Shibata, Minoru Morita, Rie Ito, Yusuke Iwasaki, Hiroshi Akiyama

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Food chemistry: X, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
2.7field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Masayoshi TomikiMorinaga Institute of Biological Science, Inc., 2-1-1 Shimosueyoshi, Tsurumi-Ku, Yokohama 230 -8504, Japan.
Masatoshi SakaiMorinaga Institute of Biological Science, Inc., 2-1-1 Shimosueyoshi, Tsurumi-Ku, Yokohama 230 -8504, Japan.
Daichi TanakaMorinaga Institute of Biological Science, Inc., 2-1-1 Shimosueyoshi, Tsurumi-Ku, Yokohama 230 -8504, Japan.
Mai HosoyaDepartment of Analytical Chemistry, School of Pharmacy and Pharmaceutical Sciences, Hoshi University, 2-4-41 Ebara, Shinagawa-Ku, Tokyo 142-8501, Japan.
Kana UchidaDepartment of Analytical Chemistry, School of Pharmacy and Pharmaceutical Sciences, Hoshi University, 2-4-41 Ebara, Shinagawa-Ku, Tokyo 142-8501, Japan.
Haruki ShibataMorinaga Institute of Biological Science, Inc., 2-1-1 Shimosueyoshi, Tsurumi-Ku, Yokohama 230 -8504, Japan.
Minoru MoritaMorinaga Institute of Biological Science, Inc., 2-1-1 Shimosueyoshi, Tsurumi-Ku, Yokohama 230 -8504, Japan.
Rie ItoDepartment of Analytical Chemistry, School of Pharmacy and Pharmaceutical Sciences, Hoshi University, 2-4-41 Ebara, Shinagawa-Ku, Tokyo 142-8501, Japan.
Yusuke IwasakiDepartment of Analytical Chemistry, School of Pharmacy and Pharmaceutical Sciences, Hoshi University, 2-4-41 Ebara, Shinagawa-Ku, Tokyo 142-8501, Japan.
Hiroshi AkiyamaDepartment of Analytical Chemistry, School of Pharmacy and Pharmaceutical Sciences, Hoshi University, 2-4-41 Ebara, Shinagawa-Ku, Tokyo 142-8501, Japan.
Hoshi University · JPMorinaga Institute of Biological Science · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Sesame is a frequent cause of adverse food reactions in allergic patients. We developed a novel sandwich enzyme-linked immunosorbent assay (ELISA) using two monoclonal antibodies and a unique extraction buffer for the detection and quantification of sesame proteins in processed foods and in raw food ingredients to clarify the validity of sesame labeling and for precautionary allergen labeling. The developed sandwich ELISA method is highly specific for sesame proteins. The limit of detection (LOD) and limit of quantification (LOQ) are 0.013 µg/g and 0.025 µg/g, respectively. The recoveries for incurred food samples, such as dressing, breads, sauce and pudding, ranged from 67 % to 81 %, while the repeatability and reproducibility coefficients of variation were less than 4.7 % and 4.5 %, respectively. The developed method has applicability for food products and is a reliable tool for the detection of hidden sesame proteins in raw food ingredients and in processed foods.

Indexed as

ELISAExtractionFood allergenIncurred foodsMonoclonal antibodySesame

Identifiers

PMID38420508
PMCPMC10900753
OpenAlexW4391848403

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.