Evidence map›Paper›PMID 38419080›Full record

ArticleMicrobial cell factories2024

Set-up of a pharmaceutical cell bank of Magnetospirillum gryphiswaldense MSR1 magnetotactic bacteria producing highly pure magnetosomes.

Théo Chades, Raphaël Le Fèvre, Imène Chebbi, Karine Blondeau, François Guyot, Edouard Alphandéry

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbial cell factories, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.9field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Théo ChadesNanobacterie SARL, 36 Boulevard Flandrin, 75116, Paris, France.
Raphaël Le FèvreNanobacterie SARL, 36 Boulevard Flandrin, 75116, Paris, France.
Imène ChebbiNanobacterie SARL, 36 Boulevard Flandrin, 75116, Paris, France.
Karine BlondeauInstitut de biologie intégrative de la cellule, UMR 9198, Université Paris Saclay, 1 Av. de la Terrasse, 91198, Gif sur Yvette, France.
François GuyotInstitut de minéralogie de physique des matériaux et de cosmochimie UMR 7590, Sorbonne Université, Université Pierre et Marie Curie, Muséum National d'Histoire Naturelle, 4 Place Jussieu, 75005, Paris, France.
Edouard AlphandéryNanobacterie SARL, 36 Boulevard Flandrin, 75116, Paris, France. edouardalphandery@hotmail.com.
École d'Ingénieurs en Chimie et Sciences du Numérique · FRInstitut de Biologie Intégrative de la Cellule · FRSorbonne Université · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We report the successful fabrication of a pharmaceutical cellular bank (PCB) containing magnetotactic bacteria (MTB), which belong to the Magnetospirillum gryphiswaldense MSR1 species. To produce such PCB, we amplified MTB in a minimal growth medium essentially devoid of other heavy metals than iron and of CMR (Carcinogenic, mutagenic and reprotoxic) products. The PCB enabled to acclimate MTB to such minimal growth conditions and then to produce highly pure magnetosomes composed of more than 99.9% of iron. The qualification of the bank as a PCB relies first on a preserved identity of the MTB compared with the original strain, second on genetic bacterial stability observed over 100 generations or under cryo-preservation for 16 months, third on a high level of purity highlighted by an absence of contaminating microorganisms in the PCB. Furthermore, the PCB was prepared under high-cell load conditions (9.10

Indexed as

MagnetosomesMagnetospirillumBacterial ProteinsIronPharmaceutical PreparationsBacterial ProteinsIronPharmaceutical PreparationsCell BankFed-batch cultivationMagnetosomeMagnetospirillum gryphiswaldense MSR1Magnetotactic bacteriumMinimal mediumPharmaceutical grade nanoparticleScale-up

Identifiers

PMID38419080
PMCPMC10903015
OpenAlexW4392241995

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.