Evidence map›Paper›PMID 38416162›Full record

ArticleJournal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology2024

Effects of serta and sertb knockout on aggression in zebrafish (Danio rerio).

Michael Tea, Yihang Kevin Pan, Joshua G R Lister, Steve F Perry, Kathleen M Gilmour

Abstract read
PubMed Publisher
In one paragraph

Article in Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.3field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Michael TeaDepartment of Biology, University of Ottawa, 30 Marie Curie Pvt, Ottawa, ON, K1N 6N5, Canada.
Yihang Kevin PanDepartment of Biology, University of Ottawa, 30 Marie Curie Pvt, Ottawa, ON, K1N 6N5, Canada.ORCID http://orcid.org/0000-0003-1455-1439
Joshua G R ListerDepartment of Biology, University of Ottawa, 30 Marie Curie Pvt, Ottawa, ON, K1N 6N5, Canada.
Steve F PerryDepartment of Biology, University of Ottawa, 30 Marie Curie Pvt, Ottawa, ON, K1N 6N5, Canada.ORCID http://orcid.org/0000-0002-8842-3766
Kathleen M GilmourDepartment of Biology, University of Ottawa, 30 Marie Curie Pvt, Ottawa, ON, K1N 6N5, Canada. kgilmour@uottawa.ca.ORCID http://orcid.org/0000-0001-9056-4704
University of Ottawa · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Zebrafish (Danio rerio) are unusual in having two paralogues of the serotonin re-uptake transporter (Sert), slc6a4a (serta) and slc6a4b (sertb), the transporter that serves in serotonin re-uptake from a synapse into the pre-synaptic cell or in serotonin uptake from the extracellular milieu into cells in the peripheral tissues. To address a knowledge gap concerning the specific roles of these paralogues, we used CRISPR/Cas9 technology to generate zebrafish knockout lines predicted to lack functional expression of Serta or Sertb. The consequences of loss-of-function of Serta or Sertb were assessed at the gene expression level, focusing on the serotonergic signalling pathway, and at the behaviour level, focusing on aggression. Whereas serta mRNA was expressed in all tissues examined, with high expression in the heart, gill and brain, only the brain displayed substantial sertb mRNA expression. In both serta

Indexed as

AggressionSerotonin Plasma Membrane Transport ProteinsZebrafishZebrafish ProteinsAnimalsAnimals, Genetically ModifiedBehavior, AnimalBrainCRISPR-Cas SystemsFemaleGene Knockout TechniquesMaleSerotoninSerotoninSerotonin Plasma Membrane Transport ProteinsZebrafish Proteins5-HT5-HT receptorAggressionMirror testMonoamine oxidaseTryptophan hydroxylase

Identifiers

PMID38416162
OpenAlexW4392242043

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.