Evidence map›Paper›PMID 38412342›Full record

ArticleThe Journal of infectious diseases2024

Uncovering the Bronchoalveolar Single-Cell Landscape of Patients With Pulmonary Tuberculosis With Human Immunodeficiency Virus Type 1 Coinfection.

Guohui Xiao, Waidong Huang, Yu Zhong, Min Ou, Taosheng Ye, Zhifeng Wang, Xuanxuan Zou, Feng Ding, Yuan Yang, Zhe Zhang and 6 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of infectious diseases, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
2.3field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 6 institutions in 3 countries.

Guohui XiaoNational Clinical Research Center for Infectious Diseases, Shenzhen Third People's Hospital, Southern University of Science and Technology, Shenzhen.
Waidong HuangBGI Research, Shenzhen.
Yu ZhongBGI Research, Shenzhen.
Min OuNational Clinical Research Center for Infectious Diseases, Shenzhen Third People's Hospital, Southern University of Science and Technology, Shenzhen.
Taosheng YeNational Clinical Research Center for Infectious Diseases, Shenzhen Third People's Hospital, Southern University of Science and Technology, Shenzhen.
Zhifeng WangBGI Research, Shenzhen.
Xuanxuan ZouBGI Research, Shenzhen.
Feng DingNational Clinical Research Center for Infectious Diseases, Shenzhen Third People's Hospital, Southern University of Science and Technology, Shenzhen.
Yuan YangBGI Research, Shenzhen.
Zhe ZhangMGI Tech, Shenzhen.
Chuanyu LiuBGI Research, Shenzhen.
Aimei LiuDepartment of Tuberculosis, Guangxi Chest Hospital, Liuzhou.
Longqi LiuBGI Research, Shenzhen.
Shuihua LuNational Clinical Research Center for Infectious Diseases, Shenzhen Third People's Hospital, Southern University of Science and Technology, Shenzhen.
Liang WuBGI Research, Shenzhen.
Guoliang ZhangNational Clinical Research Center for Infectious Diseases, Shenzhen Third People's Hospital, Southern University of Science and Technology, Shenzhen.
BGI ResearchNational Clinical Research · USSouthern University of Science and Technology · CNBotswana Geoscience Institute · BWShenzhen Third People’s Hospital · CNUniversity of Chinese Academy of Sciences · CN

Funding

China National GeneBankGuangdong Science Fund for Distinguished Young Scholars 440300231181537705092Guangxi Scientific and Technological Foundation AA22096027National Key Research and Development Plan 2021YFA1300902National Natural Science Foundation of China 82170009Shenzhen Key Laboratory of Single-Cell Omics ZDSYS20190902093613831Shenzhen Scientific and Technological Foundation KCXFZ20211020163545004State Key Laboratory of Respiratory Diseases Open Project SKLRD-OP-202324
6 · The paper itself

Abstract

backgroundCoinfection of human immunodeficiency virus type 1 (HIV-1) is the most significant risk factor for tuberculosis (TB). The immune responses of the lung are essential to restrict the growth of Mycobacterium tuberculosis and avoid the emergence of the disease. Nevertheless, there is still limited knowledge about the local immune response in people with HIV-1-TB coinfection.

methodsWe employed single-cell RNA sequencing (scRNA-seq) on bronchoalveolar lavage fluid from 9 individuals with HIV-1-TB coinfection and 10 with pulmonary TB.

resultsA total of 19 058 cells were grouped into 4 major cell types: myeloid cells, T/natural killer (NK) cells, B cells, and epithelial cells. The myeloid cells and T/NK cells were further divided into 10 and 11 subsets, respectively. The proportions of dendritic cell subsets, CD4+ T cells, and NK cells were lower in the HIV-1-TB coinfection group compared to the TB group, while the frequency of CD8+ T cells was higher. Additionally, we identified numerous differentially expressed genes between the CD4+ and CD8+ T-cell subsets between the 2 groups.

conclusionsHIV-1 infection not only affects the abundance of immune cells in the lungs but also alters their functions in patients with pulmonary TB.

Indexed as

Bronchoalveolar Lavage FluidCoinfectionHIV-1HIV InfectionsSingle-Cell AnalysisTuberculosis, PulmonaryAdultCD4-Positive T-LymphocytesCD8-Positive T-LymphocytesFemaleHumansKiller Cells, NaturalLungMaleMiddle AgedMycobacterium tuberculosisbronchoalveolar lavage fluidHIV-1local immunitypulmonary TBsingle-cell RNA sequencing

Identifiers

PMID38412342
PMCPMC11420811
OpenAlexW4392186918

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.