Evidence map›Paper›PMID 38411935›Full record

ArticleApplied biochemistry and biotechnology2024

High-Level Extracellular Expression of Hyaluronate Lyase HylP in Bacillus subtilis for Hyaluronan Degradation.

Lv Zhu, Shubo Li, Jia-Yu Jiang, Zhi-Yuan Yao, Qing Li, Shao-Jie Lian, Qiang Liu, Jin-Song Shi, Zheng-Hong Xu, Jin-Song Gong

Abstract read
PubMed Publisher
In one paragraph

Article in Applied biochemistry and biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.3field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Lv ZhuCollege of Light Industry and Food Engineering, Guangxi University, Daxue East Road No. 100, Nanning, 530004, People's Republic of China.
Shubo LiCollege of Light Industry and Food Engineering, Guangxi University, Daxue East Road No. 100, Nanning, 530004, People's Republic of China. shubo1207@gxu.edu.cn.
Jia-Yu JiangKey Laboratory of Carbohydrate Chemistry and Biotechnology, School of Life Sciences and Health Engineering, Ministry of Education, Jiangnan University, Lihu Avenue No. 1800, Wuxi, 214122, People's Republic of China.
Zhi-Yuan YaoKey Laboratory of Carbohydrate Chemistry and Biotechnology, School of Life Sciences and Health Engineering, Ministry of Education, Jiangnan University, Lihu Avenue No. 1800, Wuxi, 214122, People's Republic of China.
Qing LiShandong Engineering Laboratory of Sodium Hyaluronate and its Derivatives, Shandong Focusfreda Biotech Co., Ltd, Qufu, 273165, People's Republic of China.
Shao-Jie LianShandong Engineering Laboratory of Sodium Hyaluronate and its Derivatives, Shandong Focusfreda Biotech Co., Ltd, Qufu, 273165, People's Republic of China.
Qiang LiuShandong Engineering Laboratory of Sodium Hyaluronate and its Derivatives, Shandong Focusfreda Biotech Co., Ltd, Qufu, 273165, People's Republic of China.
Jin-Song ShiKey Laboratory of Carbohydrate Chemistry and Biotechnology, School of Life Sciences and Health Engineering, Ministry of Education, Jiangnan University, Lihu Avenue No. 1800, Wuxi, 214122, People's Republic of China.
Zheng-Hong XuCollege of Biomass Science and Engineering, Sichuan University, Chengdu, 610065, People's Republic of China.
Jin-Song GongKey Laboratory of Carbohydrate Chemistry and Biotechnology, School of Life Sciences and Health Engineering, Ministry of Education, Jiangnan University, Lihu Avenue No. 1800, Wuxi, 214122, People's Republic of China. jinsonggong.bio@hotmail.com.
Jiangnan University · CNGuangxi University · CNSichuan University · CN

Funding

Fundamental Research Funds for the Central Universities JUSRP22047National Natural Science Foundation of China 32171261Natural Science Foundation of Jiangsu Province BK20221082Taishan Industry Leading Talent of Shandong Province tscx202211017
6 · The paper itself

Abstract

Hyaluronate lyase (HA lyase) has potential in the industrial processing of hyaluronan. In this study, HylP, an HA lyase from Streptococcus pyogenes phage (SPB) was successfully expressed in Bacillus subtilis. To improve the extracellular enzyme activity of HylP in B. subtilis, signal peptide engineering systematic optimization was carried out, and cultured it from shake flasks and fermenters, followed by purification, characterization, and analysis of degradation products. The results showed that the replacement of the signal peptide increased the extracellular enzyme activity of HylP from 1.0 × 10

Indexed as

Bacillus subtilisHyaluronic AcidPolysaccharide-LyasesBacterial ProteinsEnzyme StabilityFermentationHydrogen-Ion ConcentrationProtein Sorting SignalsRecombinant ProteinsStreptococcus pyogenesTemperatureBacterial Proteinshyaluronate lyaseHyaluronic AcidPolysaccharide-LyasesProtein Sorting SignalsRecombinant ProteinsCharacterizationFermentationHyaluronate lyaseRecombinant expressionSignal peptide engineering

Identifiers

PMID38411935
OpenAlexW4392200364

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.