Studying Plant ER-PM Contact Site Localized Proteins Using Microscopy.

Lifan Li, Tong Zhang, Patrick J Hussey, Pengwei Wang

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

4 authors.

Lifan LiNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, China.

Tong ZhangNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, China.

Patrick J HusseyDepartment of Biosciences, Durham University, Durham, UK.

Pengwei WangNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan, China. wangpengwei@mail.hazu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

As in most eukaryotic cells, the plant endoplasmic reticulum (ER) network is physically linked to the plasma membrane (PM), forming ER-PM contact sites (EPCS). The protein complex required for maintaining the EPCS is composed of ER integral membrane proteins (e.g., VAP27, synaptotagmins), PM-associated proteins (e.g., NET3C), and the cytoskeleton. Here, we describe methods for studying EPCS structures and identifying possible EPCS-associated proteins. These include using artificially constructed reporters, GFP tagged protein expression followed by image analysis, and immunogold labelling at the ultrastructural level. In combination, these methods can be used to identify the location of putative EPCS proteins, which can aid in predicting their potential subcellular function.

Indexed as

Membrane ProteinsMicroscopyCell MembraneEndoplasmic ReticulumEukaryotic CellsMembrane ProteinsChi-square analysisEndoplasmic reticulumER-PM contact sitesImmunogold TEMN. benthamianaVAP27 family

Identifiers

PMID38411804

DOI10.1007/978-1-60761-783-9_15

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